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Genome-wide DNA accessibility maps after Sirt6 inactivation in muscle stem cells [ATAC-seq]

ABSTRACT: Genome-wide DNA accessibility maps after Sirt6 inactivation in muscle stem cells [ATAC-seq]

PROVIDER: PRJNA706937 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13863549_1.fastq.gz	Fastqsanger.gz
	SRR13863549_2.fastq.gz	Fastqsanger.gz
	SRR13863550_1.fastq.gz	Fastqsanger.gz
	SRR13863550_2.fastq.gz	Fastqsanger.gz
	SRR13863551_1.fastq.gz	Fastqsanger.gz

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Genome-wide DNA accessibility maps after Sirt6 inactivation in muscle stem cells [ATAC-seq]

Project description:Sirt6, the NAD+-dependent deacetylase, has been described to deacetylate H3K9, H3K18, and H3K56. However, analysis of the acetylation status revealed that loss of Sirt6 caused a massive increase of histone H3K56ac levels but no detectable change of histone H3K9ac and H3K18ac, indicating that SIRT6 is the dominant deacetylase for H3K56ac in muscle stem cells (MuSCs). Further, we investigate genome transposase-accessible chromatin in the absence of Sirt6 in MuSCs using high throughput sequencing (ATAC-seq).

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Project description:Sirt6, the NAD+-dependent deacetylase, has been described to deacetylate H3K9, H3K18, and H3K56. However, analysis of the acetylation status revealed that loss of Sirt6 caused a massive increase of histone H3K56ac levels but no detectable change of histone H3K9ac and H3K18ac, indicating that SIRT6 is the dominant deacetylase for H3K56ac in muscle stem cells (MuSCs). Further, we investigate genome-wide H3K56ac profiling in the absence of Sirt6 in MuSCs and mouse embryonic stem cells (mESCs) using high throughput sequencing (ChIP-seq).

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