Project description:We report the use of single cell RNA sequencing to identify differentially-expressed genes in liver macrophages from a mouse model of Noonan syndrome (NS) and highlight specific subpopulation clusters that may contribute to the associated insulin resistance phenotype.
Project description:Genes expression profile in liver macrophages isolated from wild type C57BL/6J mice fed either a high fat or normal diet for 18 weeks
Project description:By employing single-cell transcriptional profiling, we uncovered that mature hepatocytes re-activate reprogramming/progenitor-related genes (RRG) and dedifferentiate to liver progenitor-like cells (LPLC). Because we found macrophages regulated hepatocyte dedifferentiation, We also dissected the transcriptional profiling of hepatocytes in single-cell level during DDC injury after macrophage-depletion via clondronate liposomes. Besides, we uncovered the heterogeneity of liver macrophages in normal and DDC-injured liver via scRNA-seq as well.