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Auxin-degron system identifies immediate mechanisms of Oct4 [Nanog ChIP-seq]

ABSTRACT: Auxin-degron system identifies immediate mechanisms of Oct4 [Nanog ChIP-seq]

PROVIDER: PRJNA712293 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13917104.fastq.gz	Fastqsanger.gz
	SRR13917105.fastq.gz	Fastqsanger.gz
	SRR13917106.fastq.gz	Fastqsanger.gz
	SRR13917107.fastq.gz	Fastqsanger.gz

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Similar Datasets

Auxin-degron system identifies immediate mechanisms of Oct4

Project description:Auxin-degron system identifies immediate mechanisms of Oct4

| PRJNA708329 | ENA

Auxin-degron system identifies immediate mechanisms of Oct4 [Nanog ChIP-seq]

Project description:The pluripotency factor Oct4 is essential for the maintenance of naïve pluripotent stem cells in vitro and in vivo. However, the specific role of Oct4 in this process remains unknown. Here, we developed a rapid protein-level Oct4 depletion system that demonstrates that the immediate downstream response to loss of Oct4 is reduced expression of key pluripotency factors. Our data show a requirement for Oct4 for the efficient transcription of several key pluripotency factors, and suggest that expression of trophectoderm markers is a subsequent event. Additionally, we find that Nanog is competent to bind to the genome in the absence of Oct4, and this binding is in fact enhanced. Globally, however, active enhancer associated histone mark H3K27ac is depleted. Our work establishes that while Oct4 is required for the maintenance of the naïve transcription factor network, at a normal ESC level it antagonises this network through inhibition of Nanog binding

2021-06-17 | GSE168576 | GEO

Auxin-degron system identifies immediate mechanisms of Oct4 [RNA-seq]

Project description:Auxin-degron system identifies immediate mechanisms of Oct4 [RNA-seq]

| PRJNA712292 | ENA

Auxin-degron system identifies immediate mechanisms of Oct4 [H3K27ac ChIP-seq]

Project description:Auxin-degron system identifies immediate mechanisms of Oct4 [H3K27ac ChIP-seq]

| PRJNA712289 | ENA

Auxin-degron system identifies immediate mechanisms of Oct4 [RNA-seq]

2021-06-17 | GSE168577 | GEO

Auxin-degron system identifies immediate mechanisms of Oct4 [H3K27ac ChIP-seq]

2021-06-17 | GSE168575 | GEO

Auxin-degron system identifies immediate mechanisms of OCT4.

Project description:The pluripotency factor OCT4 is essential for the maintenance of naive pluripotent stem cells in vitro and in vivo. However, the specific role of OCT4 in this process remains unknown. Here, we developed a rapid protein-level OCT4 depletion system that demonstrates that the immediate downstream response to loss of OCT4 is reduced expression of key pluripotency factors. Our data show a requirement for OCT4 for the efficient transcription of several key pluripotency factors and suggest that expression of trophectoderm markers is a subsequent event. In addition, we find that NANOG is able to bind to the genome in the absence of OCT4, and this binding is in fact enhanced. Globally, however, the active enhancer-associated histone mark H3K27ac is depleted. Our work establishes that, while OCT4 is required for the maintenance of the naive transcription factor network, at a normal embryonic stem cell levels it antagonizes this network through inhibition of NANOG binding.

| S-EPMC8282470 | biostudies-literature

ChIP Seq of Oct4, Nanog, H3K27me3 in Oct4+/+ and Oct4 +/- mES cells

Project description:ChIP Seq of Oct4, Nanog, H3K27me3 in Oct4+/+ and Oct4 +/- mES cells

| PRJEB1833 | ENA

Auxin-degron system identifies immediate mechanisms of Oct4

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-17 | GSE168579 | GEO

NANOG-OCT4-SOX2 Regulatory Module in Human Embryonic Stem Cells

Project description:This SuperSeries is composed of the following subset Series: GSE34904: NANOG-OCT4-SOX2 Regulatory Module in Human Embryonic Stem Cells (dataset 1) GSE34912: NANOG-OCT4-SOX2 Regulatory Module in Human Embryonic Stem Cells (dataset 2) GSE34918: NANOG-OCT4-SOX2 Regulatory Module in Human Embryonic Stem Cells (dataset 3) GSE34920: NANOG-OCT4-SOX2 Regulatory Module in Human Embryonic Stem Cells (dataset 4) Refer to individual Series

2012-04-05 | E-GEOD-34921 | biostudies-arrayexpress

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