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Next generation sequencing for miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

ABSTRACT: Next generation sequencing for miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

PROVIDER: PRJNA713774 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13934200.fastq.gz	Fastqsanger.gz
	SRR13934195.fastq.gz	Fastqsanger.gz
	SRR13934196.fastq.gz	Fastqsanger.gz
	SRR13934197.fastq.gz	Fastqsanger.gz
	SRR13934198.fastq.gz	Fastqsanger.gz

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Next generation sequencing for miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

Project description:In this study, we proved the exercise-induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through establishing exercise animal model by treadmill running experiment. miRNA sequencing analysis identified 16 upregulated and 2 downregulated miRNAs in exercise group.

2021-06-23 | GSE168712 | GEO

Next generation sequencing for lncRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

Project description:In this study, we proved the exercise-induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through establishing exercise animal model by treadmill running experiment. lncRNA sequencing analysis identified 44 upregulated lncRNAs and 39 downregulated lncRNAs in exercise group.

2021-06-23 | GSE168711 | GEO

Next generation sequencing for lncRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

Project description:Next generation sequencing for lncRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

| PRJNA713773 | ENA

Next generation sequencing for lncRNA and miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

Project description:Next generation sequencing for lncRNA and miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

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The effect of Uphill and Downhill treadmill Running on the transcription of peripheral blood mononuclear cells in young male SD rats

Project description:Using Affymetrix GeneChip® Rat Genome 230 2.0 Array chip to screen the effect of Uphill and Downhill treadmill exercise on the key genes that affect bone mass in the peripheral blood mononuclear cells (PBMCs) of young male rats. Thirty-six male Sprague-Dawley rats were randomly assigned to a control group and both running groups of uphill and downhill (n = 12). Bone mineral density (BMD), bone mineral content (BMC) and bone microstructure of the femur were measured. PBMCs were extracted and the gene expression profile of mRNA detected.Gene Ontology and KEGG pathway analysis were used.The results showed exercise mainly activated the biological processes and cellular pathways involved in immune, inflammation and osteoclast differentiation.

2024-09-26 | GSE184823 | GEO

Transcription profiling of heart tissue from rats that have undergone treadmill exercise or have been sedentary for different lengths of time

Project description:Seventy two rats were randomized to twelve independent groups of rats (n = 6 in all groups) where half of the rats were chosen for training (treadmill exercise training 1.5 hour 5 days a week, in 1, 4, 24, 48 days and for 4 and 8 weeks) or sedentary (no training) as described in detail by Wisloff et al. (Intensity-controlled treadmill running in rats: VO(2 max) and cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2001 Mar;280(3):H1301-10.) or Kemi et al.(Aerobic fitness is associated with cardiomyocyte contractile capacity and endothelial function in exercise training and detraining. Circulation. 2004 Jun 15;109(23):2897-904. Epub 2004 Jun 1.). The rats were scarified one hour after training (1, 4, 24 and 48 days) or 24 hours after training (4 and 8 weeks). Biopsies form left ventricle was taken form all rats and stored on -80°C for preparation of RNA. The same procedures were performed for the control/sedentary rats which were sacrificed at the same time as the exercised rats.

2006-11-10 | E-TABM-166 | biostudies-arrayexpress

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Project description:This study investigates the effects of exercise and genetic predisposition on the transcriptomic profile of the pituitary gland of long-term selected marathon (DUhTP) and non-inbred (DUC) mice. In the exercise group, mice underwent treadmill training for three weeks. For the sedentary control group, mice were kept under minimal physical activities. For the 3-week training program, the mice were running five days per week (Monday to Friday) starting at age of 49 days after birth (Walz et. al. 2021). All mice used in this experiment were male and sacrificed at day 70 of life for tissue sampling.

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Project description:This study investigates the effects of exercise and genetic predisposition on the transcriptomic profile of the rectus femoris muscle of long-term selected marathon (DUhTP) and non-inbred (DUC) mice. All mice used in this experiment were male. In the exercise group, mice underwent treadmill training for three weeks. For the sedentary control group, mice were kept under minimal physical activities. For the 3-week training program, the mice were running five days per week (Monday to Friday) starting at age of 49 days after birth (Walz et. al. 2021). All mice were sacrificed at day 70 of life for tissue sampling.

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Voluntary running exercise after focal cerebral ischemia modifies astrocytic population and features

Project description:Voluntary running exercise after focal cerebral ischemia promotes functional recovery and prevents the ischemia-induced dendritic spine loss in the peri-infarct motor cortex layer 5. Neuronal morphology is affected by the perineuronal environmental change. Glia exert crucial roles in the formation of perineuronal environment, and exercise-induced changes of glial phenotype are expected. Here, we demonstrated that voluntary running exercise increased the population of newborn astrocytes in the acute phase after cerebral ischemia at late phase. Transcriptome analysis detected 10 upregulated genes and 70 downregulated genes by exercise in the ipsilateral cortex astrocytes. Gene cluster downregulated by exercise were significantly associated with neuronal morphology. The expression of Lipocalin 2, a factor of decreasing dendritic spines, tended to be decreased in the postischemic astrocytes by exercise. Our results suggest that exercise modifies the phenotype of postischemic astrocytes, which relate to the exercise-induced amelioration of postischemic dendritic spine loss.

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