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ABSTRACT: microbial diversity of calves with dampness-heat diarrhea

PROVIDER: PRJNA713974 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13956728_1.fastq.gz	Fastqsanger.gz
	SRR13956728_2.fastq.gz	Fastqsanger.gz
	SRR13956729_1.fastq.gz	Fastqsanger.gz
	SRR13956729_2.fastq.gz	Fastqsanger.gz
	SRR13956730_1.fastq.gz	Fastqsanger.gz

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plasma proteomic profiling reveals Dampness Heat Diarrhea mechanisms in calves

Project description:Dampness heat diarrhea (DHD) is the leading cause in calves with diarrhea in traditional Chinese medicine (TCM). However, the methods for detecting DHD diarrhea remain subjective or infective. To address this issue, the calves with dampness heat diarrhea were collected and the plasma proteomic was analyzed using data-independent acquisition (DIA)-mass spectrometry- based proteomics. The analysis revealed a total of 52 differentially expressed proteins that were uniquely altered in the DHD calves’ plasma, compared to the control. Bioinformatics analysis showed that these altered proteins were involved in a wide range of biological processes, such as intestinal immune network for IgA production, purine metabolism and PI3K signaling pathway, which implying their potential roles in DHD.

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Project description:Calves are highly susceptible to gastrointestinal infection with Cryptosporidium parvum (C. parvum), which can result in watery diarrhea and eventually death or impaired development. With little to no effective therapeutics, understanding the host’s microbiota and pathogen interaction at the mucosal immune system has been critical to identify and test novel control strategies. We used an experimental model of C. parvum challenge in neonatal calves to describe the clinical signs and mucosal innate immune and microbiota hallmarks in the ileum and colon during cryptosporidiosis and investigated the impact of supplemental colostrum feeding on C. parvum infection. The C. parvum challenged calves experienced clinical signs including pyrexia and diarrhea 5 days post challenge. These calves showed ulcerative neutrophil ileitis with a proteomic signature driven by inflammatory effectors, including reactive oxygen species and myeloperoxidases. Colitis was also noticed with an aggravated mucin barrier depletion and lack of full filled mucin granule in goblet cells. The C. parvum challenged calves also displayed a pronounced dysbiosis with a high prevalence of Clostridium species (spp.) and number of exotoxins, adherence factors, and secretion systems related to Clostridium spp. and other enteropathogens, including Campylobacter spp., Escherichia sp., Shigella spp., and Listeria spp. Daily supplementation with a high-quality bovine colostrum product mitigated some of the clinical signs and modulated the gut immune response and concomitant microbiota to a pattern more similar to that of healthy unchallenged calves.

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Project description:Dampness heat diarrhea (DHD) is the most common type of diarrhea in calves according to the Traditional Chinese veterinary medicine (TCVM) and its complex etiology bring great challenges to the treatment of this disease. Huangbai jianpi decoction (HBJP) is efficacy to treat DHD in calves. The therapeutic mechanism of HBJP is urgently required to be applied this decoction to the precise treatment of this syndrome in the clinic. We herein aimed to explore the mechanism of HBJP in DHD by using multi-omic analysis from DHD calves in Northwest China region. Proteomic results showed that some protein, including coagulation factor XIII B chain precursor (F13 B), alpha-1-acid glycoprotein precursor (ORM1) and polymeric immunoglobulin receptor (PIGR), had significant changes after treatment with HBJP. Metabolomics data demonstrated that the plasma of DHD calves were associated with high abundance of choline and pirbuterol, while after treated by HBJP the plasma was abundant in glycocholic acid and taurocholic acid. Bacterial gene sequence analysis showed that the feces of DHD calves had the highest abundance of Escherichia-Shigella, while after treated by HBJP the feces were more enriched in Megasphaera, Prevotella_2 and Bifidobacterium. Our study demonstrated that plasma proteins and metabolites, and gut microbiota were significantly different between before and after treated by HBJP in calves, and provided the theoretical basis for elucidating the mechanism of HBJP in the treatment of DHD.

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