Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic profiling of three-dimensional cholangiocyte spheroids long-term exposed to repetitive Clonorchis sinensis excretory-secretory products

ABSTRACT: Transcriptomic profiling of three-dimensional cholangiocyte spheroids long-term exposed to repetitive Clonorchis sinensis excretory-secretory products

PROVIDER: PRJNA714054 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptomic profiling of three-dimensional cholangiocyte spheroids long-term exposed to repetitive Clonorchis sinensis excretory-secretory products

Project description:Microarray and RNA-Seq analysis revealed that ESP-treated cholangiocyte H69 spheroids displayed global changes in gene expression compared to untreated spheroids. In ESP-treated H69 spheroids, 185 and 63 probes were found to be significantly upregulated and downregulated, respectively, corresponding to 209 genes (p < 0.01, fold change > 2).

2021-04-27 | GSE168792 | GEO

Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products.

Project description:BackgroundBiliary tract infection with the carcinogenic human liver fluke, Clonorchis sinensis, provokes chronic inflammation, epithelial hyperplasia, periductal fibrosis, and even cholangiocarcinoma. Complications are proportional to the intensity and duration of the infection. In addition to mechanical irritation of the biliary epithelia from worms, their excretory-secretory products (ESPs) cause chemical irritation, which leads to inflammation, proliferation, and free radical generation.MethodsA three-dimensional in vitro cholangiocyte spheroid culture model was established, followed by ESP treatment. This allowed us to examine the intrinsic pathological mechanisms of clonorchiasis via the imitation of prolonged and repetitive in vivo infection.ResultsMicroarray and RNA-Seq analysis revealed that ESP-treated cholangiocyte H69 spheroids displayed global changes in gene expression compared to untreated spheroids. In ESP-treated H69 spheroids, 185 and 63 probes were found to be significantly upregulated and downregulated, respectively, corresponding to 209 genes (p < 0.01, fold change > 2). RNA-Seq was performed for the validation of the microarray results, and the gene expression patterns in both transcriptome platforms were well matched for 209 significant genes. Gene ontology analysis demonstrated that differentially expressed genes were mainly classified into immune system processes, the extracellular region, and the extracellular matrix. Among the upregulated genes, four genes (XAF1, TRIM22, CXCL10, and BST2) were selected for confirmation using quantitative RT-PCR, resulting in 100% similar expression patterns in microarray and RNA-Seq.ConclusionsThese findings broaden our understanding of the pathological pathways of liver fluke-associated hepatobiliary disorders and suggest a novel therapeutic strategy for this infectious cancer.

| S-EPMC8056535 | biostudies-literature

Human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products.

Project description:Clonorchiasis is associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Although this is likely caused by adult Clonorchis sinensis and its excretory-secretory products (ESP), the precise molecular mechanisms remain obscure. To evaluate the effect of C. sinensis infection on differential gene expression in host hepatocytes, we used cDNA microarrays in human cholangiocarcinoma cells through the mimicking of C. sinensis infestation, and analyzed differential mRNA expression patterns of host cells. Keywords: Time course

2008-07-24 | GSE12213 | GEO

Clonorchis sinensis transcriptome

Project description:We conducted transcriptome sequencing of Clonorchis sinesis sub-organs (sucker, muscle, ovary and testis) with Illumina sequencing.

2013-01-30 | E-MTAB-827 | biostudies-arrayexpress

Clonorchis sinensis transcriptome

Project description:Clonorchis sinensis transcriptome

| PRJEB7279 | ENA

Clonorchis sinensis

Project description:Clonorchis sinensis Raw sequence reads

| PRJNA1142748 | ENA

Clonorchis sinensis

Project description:Clonorchis sinensis Raw sequence reads

| PRJNA563175 | ENA

Clonorchis sinensis

Project description:Clonorchis sinensis whole-genome sequencing

| PRJNA860012 | ENA

Cholangiocyte organoid culture in hydrogels from decellularized liver tissue

Project description:Culture of human cholangiocyte organoids in hydrogels derived from healthy liver extracellular matrix (LECM) extracts prepared from decellularized human livers are evaluated in an effort to establish a platform for production of cholangiocyte organoids for clinical regenerative applications. Human intrahepatic cholangiocyte organoids (ICO) grown in hydrogels made from LECM are compared those grown in mouse tumor derived basement membrane extracts (BME). Culture was performed with amino acids labeled with stable heavy isotopes to enable separation of ECM from the hydrogels from that of the produced by the cells with mass spectrometry (MS). MS data were used to evaluate the protein production of ICO comparing the different hydrogel substrates. The study also contains evaluation of the properties of the hydrogel substrates and focuses on expansion and differentiation of the ICO.

2022-04-13 | PXD028417 | Pride

Clonorchis sinensis

Project description:transcriptome of Clonorchis sinensis adult by Illumina technology

| PRJNA80037 | ENA