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Globodera pallida

ABSTRACT: Globodera pallida overview

PROVIDER: PRJNA714713 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	CBXT01.dat.gz	Other
	CBXT01.fasta.gz	Fasta.gz
	CBXT01.master.dat	Other
	SRR13979322_1.fastq.gz	Fastqsanger.gz

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Globodera pallida transcriptomics

Project description:As part of the Globodera pallida (potato cyst nematode) genome project weare profiling the transcriptome of the parasite across its life cycle usingRNA-Seq. . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

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Gene expression in populations of the potato cyst nematode Globodera pallida in diapause or quiescence following hydration or exposure to tomato root diffusate

Project description:Plant-parasitic nematodes (PPN) need to be adapted to survive in the absence of a suitable host or in hostile environmental conditions. Various forms of developmental arrest (including desiccation, cryopreservation, hatching inhibition and dauer stages) are used by PPN in order to survive these conditions and spread to other areas. Potato cyst nematodes (PCN) (Globodera pallida and G. rostochiensis) are frequently in a dessicated state unhatched nematodes within the egg dispersal unit inside the cyst. Long term survival seems to be associated primarily with species that have a very restricted host range which requires surviving unhatched in the absence of the host for extended periods of time. This paper shows fundamental changes in the response of quiescent and diapaused eggs of G.pallida to hydration and following exposure to tomato root diffusate using microarray gene expression analysis from a broad set of genes. Surprisingly, many unique genes were activated in the population of diapaused eggs. Transport activity was activated in both quiescent and diapaused eggs; however, the transport function genes were very different between them. Hydrated quiescent and diapaused eggs were markedly different indicating differences in adaptation for long term survival.

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