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ABSTRACT: Genetic diversity of Nile tilapia (Oreochromis niloticus) populations in Ethiopia

PROVIDER: PRJNA715380 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13996400_1.fastq.gz	Fastqsanger.gz
	SRR13996400_2.fastq.gz	Fastqsanger.gz
	SRR13996500_1.fastq.gz	Fastqsanger.gz
	SRR13996500_2.fastq.gz	Fastqsanger.gz
	SRR13996600_1.fastq.gz	Fastqsanger.gz

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Project description:Triple-negative breast cancer (TNBC) lacks therapeutic target and is difficult to treat. We report a cationic antimicrobial peptide (CAP), tilapia piscidin 4 (TP4), derived from Nile tilapia (Oreochromis niloticus), selectively toxic to TNBC. Here we aim to identify potential target in TNBC cell response to TP4 treatment by microarray study and to further address the role of TP4-resposive genes involved in TNBC cell death.

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Project description:The imbalance of intestinal flora can affect the immune function and structural integrity of the intestinal barrier, leading to the colonization and reproduction of opportunistic pathogenic bacteria in the intestine to become the dominant flora, eventually inducing enteritis. This study aimed to investigate whether fecal microbiota transplantation (FMT) could improve the gut barrier in Nile tilapia (Oreochromis niloticus). The experiment involved administering normal saline (NS group) and fecal microbiota (FMT group) (from the negative control group (C group)) to tilapia that had been treated with oxytetracycline hydrochloride (OTC) (M group) by gavage. A total of 300 male tilapia (mean body weight 596.65 ± 47.18 g) were used, with 180 of them being fed OTC (120 mg/kg body weight/day) for 7 days to induce intestinal oxidative stress, while the rest served as the control group. After confirmation of mild chronic enteritis, the tilapia were treated in different ways.

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