Project description:We performed a transcription factor (TF) ChIP-seq in human embryonal palatal mesenchyme (HEPM; HEPM ATCC ® CRL-1486™) cells with a polyclonal anitbody against TFAP2A ((ab52222, abcam®, Great Britain) in two replicates.
Project description:We performed 3'mRNA-seq in human embryonal palatal mesenchyme (HEPM) cells to identify genes expressed in this cellular modell of the developing palate.
Project description:Foreskin fibroblasts CRL 2091 (ATCC) were serum starved for 48 hours, and harvested at the indicated time points after switching to media with 10% FBS essentially as described (Iyer et al., 1999). RNA from all of the sampled time points were pooled as reference RNA to compare with RNA from individual time points as described (Iyer et al., 1999).
Project description:au13-04_cdtbis; cdt1_bis crl mutants and CDT1-RNAi lines have very similar macroscopic phenotypes as well as identical defects in plastid division and biogenesis. Our goal wwas to determine how much of these similarities originated from similar alterations of gene expression. Plantlets of crl mutant and CDT1-RNAi lines were grown in vitro on MS1/2 medium for 14 days. CDT1-RNAi lines were compared to the corresponding wild-type (Ws), whereas crl mutants were compared to their wild-type siblings and are in the Col0 ecotype.
Project description:We found that a novel gene LOC108168067 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line (ATCC® CRL-1581™, https://www.atcc.org/products/all/CRL-1581.aspx) was selected to test the effect of LOC108168067 overexpression on plasmablast/plasma cells. LOC108168067 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV201 (Fugene Corp., Guangzhou, China) to generate LOC108168067 protein. LOC108168067-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 μg/ml). To explore the effect of on gene expression, we determined mRNA profiles in LOC108168067-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.
Project description:Purpose: we conducted a froward genetic screen to isolate suppressors of the chloroplast-deficient crl mutant, and isolated a mutation affecting the OEP80 protein. Methods: We used EMS mutagenesis to find supressors of the crl mutant. Suppressors were phenotyped at the cellular and molecular (transcriptome) level, and OEP80 complex formation was investigated in the WT and crl mutant background. Results: a mutation in OEP80 fully restores the phenotype of the crl mutant, at the whole plant level, as well as at the cellular or transcriptome level
Project description:Immunoprecipitation of EGFR from irradiated A549 (ATCC CCL185) cells was performed in order to characterize bound mRNA species with the help of microarray analysis As control experiment, immunoprecipitation was also performed with IgG