Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PiRNA pathway is essential for generating functional oocytes in golden hamster

ABSTRACT: piRNA pathway is essential for generating functional oocytes in golden hamster

PROVIDER: PRJNA716965 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14061600.fastq.gz	Fastqsanger.gz
	SRR14061601.fastq.gz	Fastqsanger.gz
	SRR14061602.fastq.gz	Fastqsanger.gz
	SRR14061603.fastq.gz	Fastqsanger.gz
	SRR14061604.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 183

Similar Datasets

piRNA pathway is essential for generating functional oocytes in golden hamster

Project description:Piwi-interacting RNAs (piRNAs) are small RNAs predominantly expressed in germ cells that function in gametogenesis in various species. Notably, PIWI-deficient female mice are fertile and mouse oocytes express a panel of small RNAs that do not appear widely representative of mammals. Thus, the function of piRNAs in mammalian oogenesis remains largely elusive. Here, we generated PIWIL1- and MOV10L1-deficient golden hamsters and found that all female and male mutants were sterile, with severe defects in embryogenesis and spermatogenesis, respectively. PIWIL1-deficient oocytes displayed aberrant transposon accumulation and broad transcriptomic dysregulation, and maternal PIWIL1-deficient embryos had impaired zygotic genome activation (ZGA). Moreover, our results support that PIWIL1-piRNAs exert a non-redundant function in silencing endogenous retroviruses (ERVs) in oocytes. Together, our findings demonstrate that piRNAs are indispensable for generating functional germ cells in golden hamsters and show the informative value of this model species for functional and mechanistic investigations of piRNAs in gametogenesis, especially those related to female infertility.

2021-08-02 | GSE169528 | GEO

Small and long RNAs profiling of testis, developing oocytes and early embryos wild-type and Pld6-deficient golden hamster

Project description:Small and long RNAs profiling of developing oocytes and early embryos in wild-type and Pld6-deficient golden hamster females, as well as profiling of testis of wild-types and Pld6-mutents

2022-02-03 | GSE195935 | GEO

Golden hamster

Project description:Golden hamster Metagenome

| PRJNA1182071 | ENA

Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Project description:Many animals have a conserved adaptive genome defense system known as the Piwi-interacting RNA (piRNA) pathway which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes do not play a role in mammalian oocytes. Contrary to this, PIWIL1- and PIWIL3-defective golden hamsters we have produced show defects in production of functional oocytes. Mechanisms involved, however, vary between the two PIWI genes; lack of PIWIL1 has a strong impact on gene expression including hamster-specific young transposon de-silencing, while PIWIL3 deficiency has little impact on gene expression in oocytes, although DNA methylation of chromosomes was reduced to some extent. Our findings serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes including humans, which cannot be possible with mice.

2021-07-02 | GSE164356 | GEO

Transcriptional profiling of mouse and hamster oocytes

Project description:We profiled transcriptomes from mouse GV, MII oocytes and 1 cell embryos and Hamster oocytes.

2017-05-15 | GSE86470 | GEO

The transcriptome of golden hamster

Project description:The transcriptome of golden hamster

| PRJNA1006420 | ENA

Single-cell RNA sequencing of golden hamster upper respiratory tract (nasal mucosa and trachea), lung, kidney and small intestine

Project description:Full understanding of the pathophysiology of COVID-19 is critical for adequate treatment and development of vaccine and therapeutics. Although Golden hamster has been emerged as animal model of COVID-19, it is unknown how SARS-CoV-2 enters and infects targeted epithelial cells at molecular and cellular levels. Here, by applying single cell RNA sequencing in the upper respiratory tract, lung, kidney and intestine of golden hamster, we show that the expression profiles of host factors for SARS-CoV-2 infection in specific cell types are similar to that of human. These data can be applied to a larger investigation (data not provided here) into the expression patterns of host cell entry factors of SARS-CoV-2 in golden hamster organs.

2021-01-01 | GSE154564 | GEO

Lipoprotein profiles of human serum and golden hamster plasma

Project description:The lipoproteins of human serum and golden hamster plasma were deeper profiled by label-free proteomics and the shared proteins in lipoprotein particles from humans and golden hamsters were highlighted. The dynamic shared protein compositions of VLDL, LDL, and HDL from golden hamster plasma under normal and hyperlipidemic states were confirmed and compared by parallel reaction monitoring (PRM)-based targeted proteomics. This study was aimed to determine the relationship between the protein heterogeneity and functions of the three lipoproteins under normal conditions and explore the functional implication of protein dynamic change during the hyperlipidemic state.

2020-08-18 | PXD020963 |

Transcriptome analysis of mouse Cnot6l-/- preovulatory oocytes (GV), ovulated eggs (MII), and 1-cell zygotes

Project description:We profiled transcriptomes from Cnot6l deadenylase knock-out mouse GV oocytes, MII eggs and 1-cell zygotes in order to analyse its function during the oocyte-to-embryo (OET) transition. Transcriptome of wild-type golden hamster GV oocytes was also profiled.

2018-07-10 | GSE116771 | GEO

Transcriptional profiling of mouse and hamster oocytes

Project description:Transcriptional profiling of mouse and hamster oocytes

| PRJNA342001 | ENA