Project description:Sex specificity of the C. elegans metabolome
Russell N. Burkhardt1, Alexander B. Artyukhin1,3, Erin Z. Aprison2, Brian J. Curtis1, Bennett W. Fox1, Andreas H. Ludewig1, Amaresh Chaturbedi4, Oishika Panda1, Chester J. J. Wrobel1, Siu S. Lee4, Ilya Ruvinsky2, and Frank C. Schroeder1,
1Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States
2Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, United States
3Current address: Chemistry Department, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210, United States
4Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, United States
Correspondence to fs31@cornell.edu
Project description:p130Cas is a polyvalent adapter protein essential for cardiovascular development, and with a key role in cell movement. In order to identify the pathways by which p130Cas exerts its biological functions in endothelial cells we mapped the p130Cas interactome and its dynamic changes in response to VEGF using high-resolution mass spectrometry and reconstruction of protein interaction (PPI) networks with the aid of multiple PPI databases. The work presented here was based on a collaboration between University College London and University College Dublin. Dr Ian Evans (first author) and Prof. Ian Zachary (lab head), can be contacted at: Centre for Cardiovascular Biology and Medicine, Division of Medicine The Rayne Building, University College London, London WC1E 6JJ, United Kingdom. Contact details for University College Dublin collaborators can be found below.
Project description:Alfalfa seeds from Gwadar Port, Pakistan were cultivated at Central South University of Forestry and Technology under controlled conditions (28 °C, 70% RH, 12 h light-dark cycle) for two months.
Project description:RNA was extracted from mature ovules of two samples (i.e., WT and myb98) and sequenced with an Illumina Hi-seq 2000 sequencer in the Biodynamic Optical Imaging Center (BIOPIC) of Peking University followed by the analysis on the High Performance Computing Platform of the Center for Life Science.
Project description:In order to verify the CSC-like properties in 3D spheres of 293T cells as compared to monolayer, MCF-7 breast cancer cells under monolayer and sphere culture conditions were used as a control. Corresponding author: Chul Geun Kim, Department of Life Science, Hanyang University, Seoul 133-791, Korea (e-mail, cgkim@hanyang.ac.kr).
