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Genome-wide role of HSF1 in transcriptional regulation for desiccation tolerance of an anhydrobiotic cell line, Pv11

ABSTRACT: Genome-wide role of HSF1 in transcriptional regulation for desiccation tolerance of an anhydrobiotic cell line, Pv11

PROVIDER: PRJNA719134 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14130547_1.fastq.gz	Fastqsanger.gz
	SRR14130547_2.fastq.gz	Fastqsanger.gz
	SRR14130548_1.fastq.gz	Fastqsanger.gz
	SRR14130548_2.fastq.gz	Fastqsanger.gz
	SRR14130549_1.fastq.gz	Fastqsanger.gz

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Genome-wide role of HSF1 in transcriptional regulation for desiccation tolerance of an anhydrobiotic cell line, Pv11

Project description:Pv11 is an insect cell line established from Polypedilum vanderplanki with ability to survive complete desiccation if they were treated with trehalose. Previous studies showed contribution of HSF1 gene in this adaptation. To determine HSF1 regulatory network in Pv11 cells we prepared RNAseq libraries for wild type Pv11 (WT), HSF1 knoked out (Hsf1-/-), HSf1 resqued (Hsf1-/-; Hsf1-HaloTag/BlaR), and negative control for HSF1 resqued cells (Hsf1-/- HaloTag/BlaR) under normal conditions and under incubation with trehalose for 48 h. Comparison of mRNA profiles showed that HSF1 induced anhydrobiosis-related genes, especially LEA and TRX, but suppressed cellular process-related genes, for example transcription- and translation-related genes.

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Project description:Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of the bacterial pathogen Acinetobacter baumannii through hospitals on dry surfaces. Despite the potential importance of this stress response, scarce information is available describing the underlying mechanisms of A. baumannii desiccation tolerance. Here we characterize the factors influencing desiccation survival of A. baumannii. At the macroscale level, we find that desiccation tolerance is influenced by cell density, growth phase, and desiccation medium. Our transcriptome analysis indicates that desiccation represents a unique state for A. baumannii compared to commonly studied growth conditions and strongly influences pathways responsible for proteostasis. Remarkably, we find that an increase in total cellular protein aggregates, which is often considered deleterious, correlates positively with the ability of A. baumannii to survive desiccation. We show that artificially inducing protein aggregate formation increases desiccation survival, and more importantly, that proteins incorporated into cellular aggregates can retain activity. Our results suggest that protein aggregates may promote desiccation tolerance in A. baumannii through preserving and protecting proteins from damage during desiccation until rehydration occurs.

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Molecular Strategies of the Caenorhabditis elegans Dauer Larva to Survive Extreme Desiccation

Project description:Many organisms in the nature can drive themselves into an ametabolic state known as anhydrobiosis upon extreme desiccation. The nematode C. elegans is one of them. However, the anhydrobiotic ability of the worm is limited to a special developmental stage known as the dauer. Besides, the dauer larvae must be first treated by a mild desiccation stress (preconditioning) so that they gain desiccation tolerance. In this study, we investigated the differential gene expression during preconditioning in the C. elegans dauer.

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Project description:Characterizing Acinetobacter baumannii desiccation tolerance.

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Desiccation Tolerance of Arabidopsis thaliana seeds

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2022-10-13 | PXD028391 | Pride

Transcriptomic analysis of Pv11 cells derived from Polypedilum vanderplanki

Project description:RNA-Seq of Pv11 cells derived from the anhydrobiotic organism Polypedilum vanderplank, exposed to various stresses.

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Re-establishment of desiccation tolerance by PEG in germinated Arabidopsis thaliana seeds

Project description:Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with polyethylene glycol (PEG). We used microarrays to identify relevant genes in the re-establishment of desiccation tolerance by PEG.

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