RNA-Sequencing Analysis of ox-LDL-treated Peritoneal Macrophages Transcriptomes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA719411 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14136280.fastq.gz | Fastqsanger.gz | |||
| SRR14136281.fastq.gz | Fastqsanger.gz | |||
| SRR14136282.fastq.gz | Fastqsanger.gz | |||
| SRR14136283.fastq.gz | Fastqsanger.gz | |||
| SRR14136284.fastq.gz | Fastqsanger.gz |
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Project description:LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological transcriptional response, respectively. LDL induced a downregulation of genes associated with cholesterol biosynthesis while ox-LDL induced transcriptional alterations in genes related to inflammation, matrix expansion, lipid metabolism and processing, and apoptosis. Pentraxin-3 was secreted into the culture medium after RPE cells were stimulated with ox-LDL, and immunohistochemically evident in Bruch’s membrane of human macular samples with age-related macular degeneration. ARPE-19 cells exposed to 200?g/ml ox-LDL had a 38% apoptosis rate compared to less than 1% when exposed to LDL or untreated controls (p<0.0001). While LDL induced a physiologic response by RPE cells, a pathological phenotypic response was seen after treatment with oxidatively modified LDL. The transcriptional, biochemical, and functional data provide initial support of a role for the hypothesis that modified LDLs are one trigger for initiating events that contribute to the development of age-related macular degeneration. Keywords: treatment with non-treatment control
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