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Targeting microRNA-mediated gene repression limits the adipogenic conversion of skeletal muscle mesenchymal stromal cells

ABSTRACT: Targeting microRNA-mediated gene repression limits the adipogenic conversion of skeletal muscle mesenchymal stromal cells

PROVIDER: PRJNA719816 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14145547_1.fastq.gz	Fastqsanger.gz
	SRR14145547_2.fastq.gz	Fastqsanger.gz
	SRR14145548_1.fastq.gz	Fastqsanger.gz
	SRR14145548_2.fastq.gz	Fastqsanger.gz
	SRR14145549_1.fastq.gz	Fastqsanger.gz

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Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells.

Project description:Intramuscular fatty deposits, which are seen in muscular dystrophies and with aging, negatively affect muscle function. The cells of origin of adipocytes constituting these fatty deposits are mesenchymal stromal cells, fibroadipogenic progenitors (FAPs). We uncover a molecular fate switch, involving miR-206 and the transcription factor Runx1, that controls FAP differentiation to adipocytes. Mice deficient in miR-206 exhibit increased adipogenesis following muscle injury. Adipogenic differentiation of FAPs is abrogated by miR-206 mimics. Using a labeled microRNA (miRNA) pull-down and sequencing (LAMP-seq), we identified Runx1 as a miR-206 target, with miR-206 repressing Runx1 translation. In the absence of miR-206 in FAPs, Runx1 occupancy near transcriptional start sites of adipogenic genes and expression of these genes increase. We demonstrate that miR-206 mimicry in vivo limits intramuscular fatty infiltration. Our results provide insight into the underlying molecular mechanisms of FAP fate determination and formation of harmful fatty deposits in skeletal muscle.

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Targeting microRNA-mediated gene repression limits the adipogenic conversion of skeletal muscle mesenchymal stromal cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Keratinocyte Wnt5β promotes dermal adipogenesis by simultaneously regulating adipocyte precursor cell conversion and adipogenic signaling

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