Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms
Project description:In order to study the effect of light on gene expression in Schizophyllum commune, genome wide gene expression was analysed in 4-day-old monokaryotic and dikaryotic wild type colonies, grown either in light or in darkness.
Project description:The transcription factors Hom2 and Fst4 were shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the genes encoding Hom2 or Fst4 were deleted, no mushrooms formed. In this study we performed a genome-wide expression analysis on dikaryons of wild-type, delta-hom2 and delta-fst4.
Project description:Schizocommunin is a natural alkaloid produced from the Schizophyllum commune fungus. In 1994, the first case of allergic bronchopulmonary mycosis caused by the fungus was described. We sought to determine the effects of Schizocommunin treatment on the human transcriptome to identify mechanisms through which the pathogen may be causing the allergic response.
Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms 4 samples: - vegetative mycelium - stage I aggregates - stage II primordia - mature mushrooms RNA was obtained from 3 biological replicates and pooled
Project description:The transcription factors Hom2 and Fst4 were shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the genes encoding Hom2 or Fst4 were deleted, no mushrooms formed. In this study we performed a genome-wide expression analysis on dikaryons of wild-type, delta-hom2 and delta-fst4. A total of three samples were analyzed. One wild-type and two deletion strains (delta-hom2 and delta-fst4)
Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms. In order to study the effects of absence of expression of sc3 and sc4, genome-wide expression was analysed in a dikaryon in which those genes have been inactivated by targeted gene deletion.