Project description:Protein methyltransferases can recognise their substrates through linear sequence motifs and determination of these motifs is critical to understand methyltransferase mechanism, function and drug targeting. Here we describe MT-MAMS (methyltransferase motif analysis by mass spectrometry), a quantitative approach to characterise methyltransferase substrate recognition motifs and enzyme processivity. We validated MT-MAMS by application to lysine methyltransferase G9a, then determined the recognition motifs of Efm1 and major arginine methyltransferases Hmt1 and PRMT1.
