Project description:Early-flowering germplasm XH04 (E) and late-flowering germplasm XH05 (L) were screened. Panicle samples were collected at booting stage (B), heading stage (H) and flowering stage (F) for proteome sequencing
Project description:Transcriptional profiling of cotton fiber cells from two cotton germplasm lines, MD 52ne and MD 90ne. Comparison of fiber cell transcription profiles is between the two germplasm lines and over a developmental time-course from 8 to 24 days post anthesis in four day intervals. Cotton plants grown in 3-4 row plots of approximately 300-400 individual plants. Bulked fiber samples from multiple plants per each plot represented a biological replication. There were 3-4 spatially distinct plots per cotton germplasm line. Loop microarray hybridization experimental design. Biological replicates: 2 for each germplasm line at each time-point. Technical replicates: 2 for each germplasm line at each time-point (dye-swap).
Project description:The cell fate of primordial germ cells (PGCs) in zebrafish is pre-determined by maternally deposited germplasm, which is packaged into ribonucleoprotein complex in oocytes and, after fertilization, inherited into PGCs promoting germline fate in embryos. However, the maternal factors regulating the assembly of germplasm and PGC development remain poorly understood. In this study, we report that the maternal transcription factor Znf706 regulates the assembly of germplasm factors into a granule-like structure localized perinuclearly in PGCs during migration. Maternal and zygotic mutants of znf706 (MZznf706) exhibited deficient germplasm at the early embryonic stage, decreased PGC numbers with ectopic PGC locations during PGC migration, and lower female ratio in adulthood. Notably, the implementation of Znf706 CUT&Tag and RNA-seq on immature oocytes uncovered that Znf706 in stage I oocytes may act as a transcriptional activator and mediate mRNA binding, translational regulation, and metabolic pathways of oocytes. Hence, we propose that Znf706 is crucial for germplasm assembly and PGC development in zebrafish.
Project description:Transcriptional profiling of cotton fiber cells from two cotton germplasm lines, MD 52ne and MD 90ne. Comparison of fiber cell transcription profiles is between the two germplasm lines and over a developmental time-course from 8 to 24 days post anthesis in four day intervals.
Project description:Stylo (Stylosanthes spp.) is a dominant leguminous forage, widely cultivated in tropical and subtropical areas. Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive diseases that limit the yield of stylo. Therefore, screening resistance varieties and improving the resistance of stylo are crucial strategies to control stylo anthracnose. In this study, the resistance evaluation of main variety RY2 and 39 stylo germplasms against C. gloeosporioides was performed, 12 stylo germplasms were rated as highly resistant (HR). Among them, 2001-84 was considered to be the most disease-resistant germplasm. Then, the stylo phenotype and the infection process of C. gloeosporioides revealed that the disease severity in resistant germplasm 2001-84 was significantly milder than susceptible germplasm RY2, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR) and total antioxidant capacity (T-AOC) were higher than those in RY2 at different time post inoculation. Phosphoproteomics revealed that differentially phosphoproteins between 2001-84 and RY2 were significantly enriched in oxidoreductase activity at 96 hpi. An E3 ubiquitin ligase, SgATL31, were identified in both phosphoproteomics and plasma membrane proteomics, and overexpression of SgATL31 enhanced resistance to C. gloeosporioides in Arabidopsis. Additionally, SgATL31 promoted the accumulation of ROS in Arabidopsis leaves and stylo protoplasts under chitin treatment. Overall, this study identified the disease-resistant germplasm 2001-84 and elucidated that SgATL31 enhances resistance to anthracnose in Arabidopsis by inducing the accumulation of ROS. This study provided a reference for further exploring the functions of SgATL31 in C. gloeosporioides infection.
Project description:Stylo (Stylosanthes spp.) is a dominant leguminous forage, widely cultivated in tropical and subtropical areas. Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive diseases that limit the yield of stylo. Therefore, screening resistance varieties and improving the resistance of stylo are crucial strategies to control stylo anthracnose. In this study, the resistance evaluation of main variety RY2 and 39 stylo germplasms against C. gloeosporioides was performed, 12 stylo germplasms were rated as highly resistant (HR). Among them, 2001-84 was considered to be the most disease-resistant germplasm. Then, the stylo phenotype and the infection process of C. gloeosporioides revealed that the disease severity in resistant germplasm 2001-84 was significantly milder than susceptible germplasm RY2, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR) and total antioxidant capacity (T-AOC) were higher than those in RY2 at different time post inoculation. Phosphoproteomics revealed that differentially phosphoproteins between 2001-84 and RY2 were significantly enriched in oxidoreductase activity at 96 hpi. An E3 ubiquitin ligase, SgATL31, were identified in both phosphoproteomics and plasma membrane proteomics, and overexpression of SgATL31 enhanced resistance to C. gloeosporioides in Arabidopsis. Additionally, SgATL31 promoted the accumulation of ROS in Arabidopsis leaves and stylo protoplasts under chitin treatment. Overall, this study identified the disease-resistant germplasm 2001-84 and elucidated that SgATL31 enhances resistance to anthracnose in Arabidopsis by inducing the accumulation of ROS. This study provided a reference for further exploring the functions of SgATL31 in C. gloeosporioides infection.