Project description:Mice transplanted with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs in addition to the sgEzh2 develop myeloid leukemia with a significantly earlier onset compared to missing sgLuc. We used global expression analysis by microarray to determine regulated gene programs after addition of the Ezh2 sgRNA.
Project description:To decipher the role of Ezh2 mutations in leukemia we transplanted mice secondarily with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs with or without sgEzh2 to observe development of myeloid leukemia. Whole BM was used for ChIP-Seq analysis of histone modifications.
Project description:To elucidate the mutational interplay of Ezh2, Runx1 and Bcor we transplanted mice transplanted with LSKs transduced with sgRNAs in all combinations and observed them for development of myeloid leukemia and subsequent expression analysis of whole bone marrow by RNA-Seq.
Project description:In this experiment, we analyzed the gene expression profiles of SPOP mutant (F133V)-transduced (and vector control) RM-1-BM mouse prostate cancer cells and RAW264.7 macrophage cells in transwell coculture and monoculture in the context of olaparib (or vehicle) treatment by RNA sequencing.
Project description:RNA-Seq after Cas9-gRNA transfection with different length gRNAs we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2