Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq data for murine BM Tx transduced with different sgRNA combinations [RNA-seq]

ABSTRACT: RNA-Seq data for murine BM Tx transduced with different sgRNA combinations [RNA-seq]

PROVIDER: PRJNA725839 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14343959_1.fastq.gz	Fastqsanger.gz
	SRR14343959_2.fastq.gz	Fastqsanger.gz
	SRR14343960_1.fastq.gz	Fastqsanger.gz
	SRR14343960_2.fastq.gz	Fastqsanger.gz
	SRR14343961_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

RNA-Seq data for murine BM Tx transduced with different sgRNA combinations [RNA-seq]

Project description:To elucidate the mutational interplay of Ezh2, Runx1 and Bcor we transplanted mice transplanted with LSKs transduced with sgRNAs in all combinations and observed them for development of myeloid leukemia and subsequent expression analysis of whole bone marrow by RNA-Seq.

2023-04-30 | GSE173496 | GEO

Expression data from murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [array]

Project description:Expression data from murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [array]

| PRJNA723866 | ENA

Genome-wide maps of chromatin state from secondary murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [ChIP-seq]

Project description:Genome-wide maps of chromatin state from secondary murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [ChIP-seq]

| PRJNA723864 | ENA

Expression data from murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [array]

Project description:Mice transplanted with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs in addition to the sgEzh2 develop myeloid leukemia with a significantly earlier onset compared to missing sgLuc. We used global expression analysis by microarray to determine regulated gene programs after addition of the Ezh2 sgRNA.

2023-04-30 | GSE173135 | GEO

Genome-wide maps of chromatin state from secondary murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [ChIP-seq]

Project description:To decipher the role of Ezh2 mutations in leukemia we transplanted mice secondarily with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs with or without sgEzh2 to observe development of myeloid leukemia. Whole BM was used for ChIP-Seq analysis of histone modifications.

2023-04-30 | GSE173133 | GEO

Comparison of endogenous tRNA expression levels from cells expressing and sgRNA from either a U6 or tRNA promoter

Project description:The goal of this study was to compare the endogenous tRNA expression levels from cells transduced with a lentiviral vector encopding Cas9 and an sgRNA from a U6 or a Glutamine tRNA promoter. Using high-throughput sequencing methods (Illumina Hi-Seq 2000) we show that endogenous tRNA expression levels are not perturbed by expression of an sgRNA from a human tRNA. tRNA expression profiles were generated from 2 different transduced polyconal cell lines.

2015-09-09 | E-GEOD-69720 | biostudies-arrayexpress

Next Generation Sequencing analysis using SPOP mutant (F133V)-transduced RM-1-BM mouse prostate cancer cells and RAW264.7 mouse macrophage cells

Project description:In this experiment, we analyzed the gene expression profiles of SPOP mutant (F133V)-transduced (and vector control) RM-1-BM mouse prostate cancer cells and RAW264.7 macrophage cells in transwell coculture and monoculture in the context of olaparib (or vehicle) treatment by RNA sequencing.

2023-08-07 | GSE240199 | GEO

Project description:bm Raw sequence reads

| PRJNA886391 | ENA

Comparison of endogenous tRNA expression levels from cells expressing and sgRNA from either a U6 or tRNA promoter

2015-09-09 | GSE69720 | GEO

Screening of a heptamer-type sgRNA library for therapeutic agents against hematological malignancies.

Project description:tRNase ZL-utilizing efficacious gene silencing (TRUE gene silencing) is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the properties of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA) and that cytosolic tRNase ZL can modulate gene expression by cleaving mRNA under the direction of cellular 5M-bM-^@M-2-half-tRNA or microRNA as sgRNA. In order to estimate a number of potential therapeutic heptamer-type sgRNAs for hematological malignancies, we constructed an sgRNA library composed of 156 heptamer-type sgRNAs, and examined how the sgRNAs affect viability of leukemia and myeloma cells. And we found that 20 of the 156 sgRNAs can efficiently induce apoptosis in at least one of the cancer cell lines. Furthermore, we demonstrated that 4 of the 20 effective sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models. DNA microarray analysis for changes in an mRNA profile by these four heptamer-type sgRNAs suggested at least one candidate target mRNA that contains a potential tRNase ZL target site for each sgRNA. Changes in gene expression in HL60 cells were measured after 18-hour incubation in the absence or presence of one of five different heptamer-type sgRNAs. *Heptamer sequences requested but not provided by submitter

2014-06-30 | E-GEOD-49578 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data