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RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex

ABSTRACT: RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex

PROVIDER: PRJNA725407 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12611964_1.fastq.gz	Fastqsanger.gz
	SRR12611964_2.fastq.gz	Fastqsanger.gz
	SRR12611965_1.fastq.gz	Fastqsanger.gz
	SRR12611965_2.fastq.gz	Fastqsanger.gz
	SRR12611966_1.fastq.gz	Fastqsanger.gz

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RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex [2D NSCs]

Project description:RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex [2D NSCs]

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RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex [3D cerebral organoid]

Project description:RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex [3D cerebral organoid]

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Project description:Use cerebral organoid and mouse and mouse models to study the function of RGCC in neocortical development.

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RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex [3D cerebral organoid]

Project description:Use cerebral organoid and mouse and mouse models to study the function of RGCC in neocortical development.

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RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcriptomes of germinal zones of human and mouse fetal neocortex suggest a role of extracellular matrix in progenitor self-renewal.

Project description:The expansion of the neocortex during mammalian brain evolution results primarily from an increase in neural progenitor cell divisions in its two principal germinal zones during development, the ventricular zone (VZ) and the subventricular zone (SVZ). Using mRNA sequencing, we analyzed the transcriptomes of fetal human and embryonic mouse VZ, SVZ and cortical plate (CP). We describe sets of genes that are up- or down-regulated in each germinal zone. These data suggest that cell adhesion and cell-extracellular matrix (ECM) interactions promote the proliferation and self-renewal of neural progenitors in the developing human neocortex. Notably, relevant ECM-associated genes include distinct sets of collagens, laminins, proteoglycans and integrins, along with specific sets of growth factors and morphogens. Our data establish a basis for identifying novel cell-type markers and open up avenues to unravel the molecular basis of neocortex expansion during evolution. Total RNA was isolated from the VZ, inner SVZ (ISVZ), outer SVZ (OSVZ) and CP of six 13-16 weeks post-conception (w.p.c.) human fetuses and from the VZ, SVZ and CP of five E14.5 mouse embryos using laser capture microdissection of Nissl-stained cryosections of dorsolateral telencephalon. Poly A+ RNA was used as template for the preparation of cDNA which were then subjected to single-end 76-bp RNA-Seq.

2012-07-02 | E-GEOD-38805 | biostudies-arrayexpress

Transcriptomes of germinal zones of human and mouse fetal neocortex suggest a role of extracellular matrix in progenitor self-renewal.

2012-07-03 | GSE38805 | GEO

de novo DNA Methylation Balances Hematopoietic Stem Cell Self-Renewal and Differentiation

Project description:Cytosine methylation is an epigenetic mark usually associated with gene repression. Despite a requirement for de novo DNA methylation for differentiation of embryonic stem cells, its role in somatic stem cells is unknown. Using conditional ablation, we show that loss of either, or both, Dnmt3a or Dnmt3b, progressively impedes hematopoietic stem cell (HSC) differentiation during serial in vivo passage. Concomitantly, HSC self-renewal is immensely augmented in absence of either Dnmt3, particularly Dnmt3a. Dnmt3-KO HSCs show upregulation of HSC multipotency genes and downregulation of early differentiation factors, and the differentiated progeny of Dnmt3-KO HSCs exhibit hypomethylation and incomplete repression of HSC-specific genes. HSCs lacking Dnmt3a manifest hyper-methylation of CpG islands and hypo-methylation of genes which are highly correlated with human hematologic malignancies. These data establish that aberrant DNA methylation has direct pathologic consequences for somatic stem cell development, leading to inefficient differentiation and maintenance of a self-renewal program. Reduced representation bisulfite sequencing (MspI,~40-220bp size fraction) of secondarily-transplanted wild-type and Dnmt3a conditional knockout hematopoietic stem cells. We used microarrays to detail the global expression of genes in secondarily-transplanted control-HSCs and Dnmt3a-KO-HSCs.

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de novo DNA Methylation Balances Hematopoietic Stem Cell Self-Renewal and Differentiation

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2021-03-01 | GSE161690 | GEO

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