Project description:Diel proteomes of Trichodesmium colonies sampled from the field on March 10, 2018 from the Eastern subtropical Atlantic at 65 22.420W 17 0.284 N. These proteomes inform a broad study of diel proteome oscillations in Trichodesmium that support simultaneous photosynthesis and nitrogen fixation during the day.
Project description:Metaproteomes of individual Trichodesmium colonies collected from a single location in the Carribbean sea (65.22W, 17.02N) at 17:00 local time. Some colonies were associated with auto-fluorescent mineral particles. Their proteomes were analyzed individually to investigate the effect of the minerals on colony physiology.
Project description:The filamentous diazotrophic cyanobacteria Trichodesmium spp. supply fixed nitrogen (N) to the N-depleted oligotrophic oceans where their growth is often limited by the low availability of phosphorus(P) and/or iron. Previous studies have mostly been focused on the effects of ocean acidification on Trichodesmium under nutrient sufficient or iron-limited conditions. Only a few studies have examined the impacts of ocean acidification on Trichodesmium grown at low P concentrations using non-steady-state batch cultures. Here we cultured Trichodesmium using P-limited continuous cultures (chemostat) to mimic steady-state oceanic low P condition, and used comparative NGS-derived Trichodesmium transcriptome profiling (RNA-seq) analysis to find differentially expressed genes and cellular pathways in response to acidification.
2022-10-13 | GSE181428 | GEO
Project description:2018 Helgoland Spring Bloom Metagenomes