Project description:Transcriptional profiling of M. tuberculosis growing in log phase treated with various concentrations of carbon monoxide versus untreated controls Keywords: Dose response
Project description:Transcriptional profiling of M. tuberculosis growing in log phase treated with various concentrations of carbon monoxide versus untreated controls Keywords: Dose response Two condition experiment, CO treated cells at 2000, 200 and 20 ppm versus untreated controls. Biological replicates: 2 replicates per dose, 1 array per replicate
Project description:PTEN encodes a tumor suppressor with lipid and protein phosphatase activities whose dysfunction has been implicated in melanomagenesis; less is known about how its phosphatases regulate melanoma metastasis. We show that PTEN expression negatively correlates with metastatic progression in human melanoma samples and a PTEN-deficient mouse melanoma model. Wildtype PTEN expression inhibited melanoma cell invasiveness and metastasis in a dose-dependent manner, behaviors that specifically required PTEN protein phosphatase activity. PTEN phosphatase activity regulated metastasis through Entpd5. Entpd5 knockdown reduced metastasis and IGF1R levels while promoting ER stress. In contrast, Entpd5 overexpression promoted metastasis and enhanced IGF1R levels, while reducing ER stress. Moreover, Entpd5 expression was regulated by the ER stress sensor ATF6. Altogether, our data show that PTEN phosphatase activity inhibits metastasis by negatively regulating the Entpd5/IGF1R pathway through ATF6, thereby identifying novel candidate therapeutic targets for the treatmtreatingwith PTEN mutant melanoma.
Project description:Investigating the role of carbon monoxide and a CO sensor protein CooA in the physiology of Desulfovibrio vulgaris Hildenborough using whole genome expression analysis Comparison of whole genome expression changes in the wild type and a strain deleted for CooA (DVU2097) in the presence and absence of carbon monoxide
Project description:Investigating the role of carbon monoxide and a CO sensor protein CooA in the physiology of Desulfovibrio vulgaris Hildenborough using whole genome expression analysis
Project description:This SuperSeries is composed of the following subset Series: GSE11095: Dose response of carbon monoxide treatment of M. tuberculosis GSE11096: Role of M. tuberculosis dosS and dosT in CO sensing Refer to individual Series