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Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq analysis of PDX tumors)

ABSTRACT: Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq analysis of PDX tumors)

PROVIDER: PRJNA727988 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14463669_1.fastq.gz	Fastqsanger.gz
	SRR14463669_2.fastq.gz	Fastqsanger.gz
	SRR14463670_1.fastq.gz	Fastqsanger.gz
	SRR14463670_2.fastq.gz	Fastqsanger.gz
	SRR14463671_1.fastq.gz	Fastqsanger.gz

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Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator

Project description:Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator

| PRJNA726876 | ENA

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq)

Project description:Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq)

| PRJNA727986 | ENA

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (CUT&RUN)

Project description:Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (CUT&RUN)

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Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (SLAM-Seq)

Project description:Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (SLAM-Seq)

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Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq analysis of PDX tumors)

Project description:Herpesviruses rely on host cell transcription and translation machineries for replication. Viral proteins thus function to redirect multiple cellular proteins for viral replication. In herpesvirus replicating cells, host cell gene transcription is frequently down-regulated because important transcriptional apparatuses are appropriated by viral transcription factors. Here we show that an evolutionally-shaped viral protein sequence is a great starting material for unique drug development to modulate cellular transcription. Cellular c-Myc protein (MYC) is overexpressed in over 70% of all types of cancer cells and therefore a very attractive target to control cancer cell growth. We identified a small functional peptide derived from the Kaposi's sarcoma-associated herpesvirus transactivator (K-Rta), which strongly attenuates MYC expression, reduces cell proliferation, and selectively kills cancer cells in both tissue culture and a xenograft tumor mouse model. Mechanistically, the peptide blocks promoter-enhancer interactions by preventing coactivator complex consisting of Nuclear receptor coactivator 2, p300, and SWI/SNF proteins from engaging the MYC promoter in leukemia cells. Target gene profiling with SLAM-seq suggests that the viral peptide attenuates MYC expression through a mechanism likely different from that of BET bromodomain inhibitors. Furthermore, fusing the 13 amino acids peptide with humanized anti-CD22 single chain armed the antibody drug with cell killing ability, and inhibited cell growth in soft agar. Our studies thus demonstrate the utility of the peptide sequence as a therapeutics module, which may be used to modulate MYC activity in a cell type-specific manner.

2021-12-01 | GSE174033 | GEO

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (SLAM-Seq)

2021-12-01 | GSE174115 | GEO

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (CUT&RUN)

2021-12-01 | GSE173724 | GEO

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator (RNA-Seq)

2021-12-01 | GSE174031 | GEO

Targeting MYC Transcription with Small Peptide Derived from KSHV Transactivator

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-01 | GSE173725 | GEO

KSHV transactivator-derived small peptide traps coactivators to attenuate MYC and inhibits leukemia and lymphoma cell growth.

Project description:In herpesvirus replicating cells, host cell gene transcription is frequently down-regulated because important transcriptional apparatuses are appropriated by viral transcription factors. Here, we show a small peptide derived from the Kaposi's sarcoma-associated herpesvirus transactivator (K-Rta) sequence, which attenuates cellular MYC expression, reduces cell proliferation, and selectively kills cancer cell lines in both tissue culture and a xenograft tumor mouse model. Mechanistically, the peptide functions as a decoy to block the recruitment of coactivator complexes consisting of Nuclear receptor coactivator 2 (NCOA2), p300, and SWI/SNF proteins to the MYC promoter in primary effusion lymphoma cells. Thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq) with target-transcriptional analyses further confirm that the viral peptide directly attenuates MYC and MYC-target gene expression. This study thus provides a unique tool to control MYC activation, which may be used as a therapeutic payload to treat MYC-dependent diseases such as cancers and autoimmune diseases.

| S-EPMC8639922 | biostudies-literature

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