Ontology highlight
ABSTRACT:
PROVIDER: PRJNA729517 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14514376_1.fastq.gz | Fastqsanger.gz | |||
| SRR14514376_2.fastq.gz | Fastqsanger.gz | |||
| SRR14514377_1.fastq.gz | Fastqsanger.gz | |||
| SRR14514377_2.fastq.gz | Fastqsanger.gz | |||
| SRR14514378_1.fastq.gz | Fastqsanger.gz |
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Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
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Project description:Predatory bugs capture prey by injecting venom from their salivary glands using specialized stylets. Understanding venom function has been impeded by a scarcity of knowledge of their venom composition. We therefore examined the proteinaceous components of the salivary venom of the predatory stink bug Arma chinensis (Hemiptera: Pentatomidae). Using gland extracts and venoms from 5th-instar nymphs or adult females, we performed shotgun proteomics combined with venom gland transcriptomics. We found that the venom of A. chinensis comprised a complex suite of over a hundred individual proteins, including oxidoreductases, transferases, hydrolases, ligases, protease inhibitors, and recognition, transport and binding proteins. Besides the uncharacterized proteins, hydrolases such as venom serine proteases, cathepsins, phospholipase A2, phosphatases, nucleases, alpha-amylases, and chitinases constitute the most abundant protein families. However, salivary proteins shared by and unique to other predatory heteropterans were not detected in A. chinensis venom. Injection of the proteinaceous (> 3 kDa) venom fraction of A. chinensis gland extracts or venom into its prey, the larvae of the Oriental armyworm Mythimna separata (Walker, 1865), revealed insecticidal activity against lepidopterans. Our data expands the knowledge of heteropteran salivary proteins and suggests predatory asopine bugs as a novel source for bioinsecticides.
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