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ABSTRACT: House dust mite ITS2

PROVIDER: PRJNA729580 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14519157_1.fastq.gz	Fastqsanger.gz
	SRR14519157_2.fastq.gz	Fastqsanger.gz
	SRR14519158_1.fastq.gz	Fastqsanger.gz
	SRR14519158_2.fastq.gz	Fastqsanger.gz
	SRR14519159_1.fastq.gz	Fastqsanger.gz

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Gene Expression Changes Associated With TLR2 Mediated Inhibition of Allergen Specific TH2 Response In Vitro

Project description:Investigation of the gene expression changes associated with TLR2 adjuvant (lipoteichoic acid; LTA) inhibition of allergic TH2 responses in peripheral blood mononuclear cell cultures (derived from house dust mite allergic individuals) stimulated with house dust mite extract. Pooled samples from 5 individuals were analysed by microarray; qPCR validation was carried out in a larger cohort of 23 individuals.

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Project description:Response to allergen was studied in bronchial epithelial cell line H292. Cells were cultured and subsequently exposed to House dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostastics. We find a strong response to allergen in epithelial cells Experiment Overall Design: Bronchial epithelial cell line was cultured and stimulated with house dust mite extract or diluent alone. Both conditions were performed in triplicate

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Expression data from lung CD11b+ conventional dendritic cells in the steady-state and following exposure to house dust mite allergens

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Expression data from lungs of Vldlr-/- mice and wild type (WT) B6129SF2/J mice that had received intranasal house dust mite (HDM) challenges to induce experimental asthma or saline, as a control.

Project description:The very low density lipoprotein receptor (VLDLR) is a multi-ligand receptor that mediates pleiotropic biological processes, such as brain development. In this dataset, we include the expression data obtained from lungs from mice that had been challenged with house dust mite to induce experimental asthma or saline, as a control. These data are used to obtain genes that are differentially expressed in response to VLDLR signaling. We compared wild type and VLDLR knock out mice that had been sensitized and challenged with house dust mite or saline, as a control. There were total 16 samples with four biological relicates in each group.

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Project description:The goal of this project was to identify strain-dependent expression of miRNAs in lung tissue from Collaborative Cross founder strains that were sensitized and challenged with house dust mite allergen (Der p 1).

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Project description:To analyze lung miRNA signatures using a house dust mite model (HDM) of asthma. microRNA sequencing (miRNA-seq) from BALB/c mice (male and female, ages 6-8 weeks old) lung tissue was performed.

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