Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series
Project description:Small nucleolar RNAs (snoRNA) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs. But we found that knock down of a C/D box snoRNA, Bm-15, can induce apoptosis of insect Spodoptera frugiperda Sf9 cells. For the genome sequence of Spodoptera frugiperda is incomplete, here with the de novo sequencing method, transcriptome of Spodoptera frugiperda cell line Sf9 were sequenced after being transfected with overexpression vector and repression probes of snoRNA Bm-15. Results showed that 21 apoptosis-related genes were up-regulated upon Bm-15 inhibition and down-regulated with Bm-15 overexpression.
Project description:We report the application of whole transcriptome sequencing technology for high-throughput profiling of coding and non-coding RNAs associated with Spodoptera frugiperda feeding in Zea mays. 4,366 mRNAs and 233 lncRNAs were differentially expressed during Spodoptera frugiperda feeding in Zea mays. Our data contribute to the understanding of the function of coding and non-coding RNAs in the regulation of plant-insect interactions.
Project description:In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. Variations in gene expression were seen as per the expectation.
Project description:Purpose: We analyzed the 3rd-instar Spodoptera frugiperda response after SfAV-1a infection. Specifically, we targeted three gene types in the infected host namely, mitochondrial, cytoskeleton and innate immunity genes.
Project description:Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management, naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent in other studies. We have, herein, aimed at quantifying the concentration and at generating the total proteomic profiles of one insect host, Spodoptera frungiperda, during the progression of infection with 4 EPN and their associated bacteria, as compared to that of healthy controls. The time-course expression of larval proteins of the test insect, Spodoptera frugiperda, during the progression of larval mortality due to nematode-bacterium infection, more specifically,4 different combinations of Heterorhabditis indica-Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The proteomic profiling was facilitated through LC-MS/MS. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Spodoptera frugiperda.
Project description:Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management, naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent in other studies. We have, herein, aimed at quantifying the concentration and at generating the total proteomic profiles of one insect host, Spodoptera frungiperda, during the progression of infection with 4 EPN and their associated bacteria, as compared to that of healthy controls. The time-course expression of larval proteins of the test insect, Spodoptera frugiperda, during the progression of larval mortality due to nematode-bacterium infection, more specifically,4 different combinations of Heterorhabditis indica-Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The proteomic profiling was facilitated through LC-MS/MS. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Spodoptera frugiperda.
Project description:MicroRNAs (miRNAs) are endogenous small noncoding RNAs (18–25 nt) that are involved in many physiological processes including development, cancer, immunity, apoptosis and host-microbe interactions through posttranscriptional regulation of gene expression. In this study, we measured the profile of small RNAs in Zea mays after one day and three days of Spodoptera frugiperda feeding. We identified 500 miRNAs, including 449 known and 51 novel miRNAs. In addition, we identified the miRNAs differentially expressed in Z. mays after one day and three days of S. frugiperda feeding, and the possible target genes were identified. This study identified critical miRNAs involved in the Z. mays during S. frugiperda feeding, thus providing a useful resource for exploring the regulatory role of miRNAs during plant-insect interactions.