Project description:Firefighters are routinely exposed to polycyclic aromatic hydrocarbons (PAHs) during fire suppression activities, molecular biomarkers reflecting such exposure remain underexplored. Thus, we explored an integrated exposure assessment framework linking PAH contamination on turnout gear and skin to changes in microRNA (miRNA) expression in skin and blood biospecimens.
Project description:Effect of 5.4 ppm polycyclic aromatic hydrocarbons (PAHs) and 18.2 ppm alkylphenols (APs) on gene expression in adult Zebrafish (Danio rerio) liver after 1 and 7 weeks of water-borne exposure.
Project description:A vast range of pollutants (polycyclic aromatic hydrocarbons, heavy metals, particulate matters) are generated by increasing urbanization. The skin epidermis, as the outermost line of defense of the body, is continuously exposed to external aggressions, including environmental pollution. To study the effects of air pollutant exposure on skin physiology, the first challenge was to define a model that mimics as close as possible the in vivo skin exposure to atmospheric pollution. In the present study, the pollutant mixture was designed on the basis of the French non-profit organization ‘Air Parif’ database and nebulized on human skin explants using the Pollubox® device. Cellular and molecular modifications in skin explants were investigated from several donors exposed to a pollutant mix (P) or an organic solvent (OS). Transcriptomic analyses coupled to immunolabelling showed that P exposure induced the expression of detoxifying enzymes notably in the stratum granulosum. Interestingly, transmission electron microscopy observations demonstrated that, in our model, diesel particles smaller than 300 nm could penetrate the skin up to the granular layer. This may cause local alterations of the microenvironment which alter the skin physiology. Therefore, the response of the epidermis to pollutant exposure is likely to be layer-specific.
Project description:The mechanisms of cardiotoxicity of the three widespread model polycyclic aromatic hydrocarbons (PAHs) retene, pyrene and phenanthrene were explored in rainbow trout (Oncorhyncus mykiss) early life stages. Newly hatched larvae were exposed to sublethal doses of each individual PAH causing no detectable morphometric alterations. Changes in the cardiac proteome were assessed after 7 or 14 days of exposure to each PAH.
2020-08-06 | PXD017294 | Pride
Project description:Laboratory exposure of mangrove sediments to polycyclic aromatic hydrocarbons
Project description:This microarray experiment aimed at studying the response of Aedes aegypti 4th stage-larvae to various xenobiotics, including insecticides, polycyclic aromatic hydrocarbons, herbicides and heavy metals.
Project description:In this study, we compare genomic signature safter treatment ofprimary human bronchial epithelial cells (HBEC) cultured in 3D with polycyclic aromatic hydrocarbons (PAHs) and identify genesets predictive of cancer risk.
Project description:C57BL/6j mice were treated with polycyclic aromatic hydrocarbons (benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, and 2,3,7,8 tetrachlorodibenzo-p-dioxin) Cyp1a1 and Cyp1b1 deletion separately and in combination were evaluated relative to the intact WT mice
