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Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

ABSTRACT: Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

PROVIDER: PRJNA731503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14607572.fastq.gz	Fastqsanger.gz
	SRR14607573_1.fastq.gz	Fastqsanger.gz
	SRR14607573_2.fastq.gz	Fastqsanger.gz
	SRR14607574.fastq.gz	Fastqsanger.gz
	SRR14607575_1.fastq.gz	Fastqsanger.gz

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Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

Project description:WT or Cd226-/- mice were treated with DMBA and TPA. Three weeks later, cells were isolated from mice back skin, and divided CD45+ and CD45- cells by cell sorter. CD45+ and CD45- cells were mixed in 1:1 ratio, and we performed scRNA sequencing of skin cells.

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Single-cell RNA (10X Chromium) of mouse DMBA/TPA treated or control back skin and matched livers (1) (DKFZ) 2022

2023-11-21 | E-MTAB-12454 | biostudies-arrayexpress

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Project description:We generated single cell transcriptomes from full thickness skin biopsies in naked mole-rat to quantify the skin cell types found in this species (control samples). To study if and how naked mole-rat skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment traditionally performed in mice, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 12 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

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Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK B) 2022

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Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK A) 2022

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