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Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice.

ABSTRACT: Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice.

PROVIDER: PRJNA732689 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14648602.fastq.gz	Fastqsanger.gz
	SRR14648603.fastq.gz	Fastqsanger.gz
	SRR14648604.fastq.gz	Fastqsanger.gz
	SRR14648605.fastq.gz	Fastqsanger.gz
	SRR14648606.fastq.gz	Fastqsanger.gz

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Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [BS-seq]

Project description:Transmission of epigenetic information between generations occurs in nematodes, flies and plants, mediated by specialised small RNA pathways, modified histones and DNA methylation. In mammals, evidence supports similar trans- and inter-generational effects although the underlying mechanisms are incompletely understood. Here a luciferase knock-in reporter mouse for the imprinted Dlk1 locus was generated to visualise and track epigenetic fidelity across generations. Exposure to high-fat diet (HFD) in pregnancy provoked sustained re-expression of the normally silent maternal Dlk1 allele in offspring (a loss of imprinting) and increased DNA methylation at the sDMR. In the next generation heterogeneous Dlk1 mis-expression was seen exclusively among animals born to F1-exposed females. Oocytes from these females showed altered gene and miR expression without changes in DNA methylation, and correct imprinting was restored in subsequent generations. Our results illustrate how diet impacts the foetal epigenome, disturbing canonical and non-canonical imprinting mechanisms to modulate the properties of two successive generations of offspring.

2022-04-01 | GSE175537 | GEO

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [RNA-seq]

2022-04-01 | GSE175536 | GEO

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [BS-seq]

Project description:Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [BS-seq]

| PRJNA732781 | ENA

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [RNA-seq]

Project description:Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [RNA-seq]

| PRJNA732782 | ENA

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice.

Project description:Transmission of epigenetic information between generations occurs in nematodes, flies and plants, mediated by specialised small RNA pathways, modified histones and DNA methylation. Similar processes in mammals can also affect phenotype through intergenerational or trans-generational mechanisms. Here we generate a luciferase knock-in reporter mouse for the imprinted Dlk1 locus to visualise and track epigenetic fidelity across generations. Exposure to high-fat diet in pregnancy provokes sustained re-expression of the normally silent maternal Dlk1 in offspring (loss of imprinting) and increased DNA methylation at the somatic differentially methylated region (sDMR). In the next generation heterogeneous Dlk1 mis-expression is seen exclusively among animals born to F1-exposed females. Oocytes from these females show altered gene and microRNA expression without changes in DNA methylation, and correct imprinting is restored in subsequent generations. Our results illustrate how diet impacts the foetal epigenome, disturbing canonical and non-canonical imprinting mechanisms to modulate the properties of successive generations of offspring.

| S-EPMC9072353 | biostudies-literature

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice.

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-04-01 | GSE175538 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of epididymal adipose tissue transcriptomes from mice fed either a control-diet or aWestern-diet for one or five successive generations

Project description:Purpose: Successive paternal generations of unhealthy diet induce a fat mass increase. The goal of this study is to compare epididymal adipose tissue transcriptome profiling (RNA-seq) of mice fed either a control-Diet or a High-Fat-Diet for One or Five successive generations. The aim of the study is to identify differentially expressed RNA in epididymal adipose tissue of obese males which might be involved in the exacerbation of induced by the maintenance of High Fat Diet feeding for 5 successive generations. Methods: Epididymal adipose tissue mRNA profiles of 18-months-old mice fed either a control-Diet or a Western-Diet for One or Five successive generations were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were quantitavely analyzed. Reads abundance was evaluated for each gene followed by annotation versus mouse GTF by using the featureCounts function.The R package Edger was used in order to normalize the reads and to identify differentially expressed (DE) genes. Results: RNA-seq data revealed a specific enrichment in gene related to metabolic syndrome traits in male mice obtained after successive paternal generations of Western-Diet.

2021-04-15 | GSE148972 | GEO

Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from mice fed either a control-Diet or a Western-diet for One or Five successive generations

Project description:Gene expression analysis of sperm small RNA obtained from mice fed either a control or Western diets for one or five successive generations through the paternal lineage. The aim of the study is to identify differentially expressed small RNA in sperm of obese males which might be involved in the long-term epigenetic inheritance of obesity induced by the maintenance of High Fat Diet feeding for 5 successive generations.

2021-03-31 | GSE138989 | GEO

Characterization of the intergenerational impact of in utero and postnatal oxycodone exposure

Project description:Based on the significant physical attributes observed at P14 in the F1 and F2 generations exposed to oxycodone in utero (IUO) and postnatally (PNO), we performed RNA-Seq analysis on the nucleus accumbens (NAc). The NAc was chosen given its association with the reward pathway. RNA-Seq analysis showed several up-and down-regulated genes among the IUO, PNO, and saline groups in both the F1 and F2 generations

2020-09-03 | GSE157348 | GEO

Profiling the neuroimmune cascade in 3xTg mice exposed to successive mild traumatic brain injuries

Project description:Repetitive mild traumatic brain injuries (rmTBI) sustained within a window of vulnerability can result in long term cognitive deficits, depression, and eventual neurodegeneration associated with tau pathology, amyloid beta plaques, gliosis, and neuronal and functional loss. However, we have limited understanding of how successive injuries affect the brain to result in these devastating long-term consequences. In the current study, we addressed the question of how repeated injuries affect the brain in the acute phase of injury (<24hr) by exposing the 3xTg-AD mouse model of tau and amyloid beta pathology to successive (1x, 3x, 5x) once-daily weight drop closed-head injuries and quantifying transcription at 30min, 4hr, and 24hr after each injury.

2024-05-17 | GSE226838 | GEO

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