Project description:To reveal influence of anesthesia in toxicogenomics study, gene expression analysis of liver of Sprague-Dawley rat after isoflurane anesthesia or CO2/O2 anesthesia was performed. The liver samples were excised from each rat after isoflurane anesthesia or CO2/O2 anesthesia. The gene expression profiles in liver were measureed by Agilent microarray.
Project description:2-hydroxy-4-methoxybenzophenone (HMB) has been reported to have weak estrogenic activity by in vivo and in vitro studies, making it a chemical with potential reproductive concern. To explore if perinatal HMB exposure altered the gene expression profiling in the rat testis, we analyzed whole genome and mitochondria-related gene expression profiling on the testis ontained from Sprague-Dawley rat offspring exposed prenatally and lactationally to varying dose of HMB.
Project description:2-hydroxy-4-methoxybenzophenone (HMB) has been reported to have weak estrogenic activity by in vivo and in vitro studies, making it a chemical with potential reproductive concern. To explore if perinatal HMB exposure altered the gene expression profiling in the rat prostate, we analyzed whole genome and mitochondria-related gene expression profiling on the Prostate ontained from Sprague-Dawley rat offspring exposed prenatally and lactationally to varying dose of HMB.
Project description:We performed deep sequencing of the miRNAs present in ~20 toxicologically relevant tissues in Sprague Dawley rats and used three independent analysis of the data to determine which miRNAs are tissue specific and tissue enriched. We performed deep sequencing of miRNAs in 21 organs from 5 male Sprague Dawley rats and 23 organs from 5 female Sprague Dawley rats.
