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RNA-seq of CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

ABSTRACT: RNA-seq of CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

PROVIDER: PRJNA733470 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14676300_1.fastq.gz	Fastqsanger.gz
	SRR14676300_2.fastq.gz	Fastqsanger.gz
	SRR14676301_1.fastq.gz	Fastqsanger.gz
	SRR14676301_2.fastq.gz	Fastqsanger.gz
	SRR14676302_1.fastq.gz	Fastqsanger.gz

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RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

Project description:RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

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RNA-seq of CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

Project description:CD4+ memory T cells play a pivatol role in mediating long-term immunity and therefore are an important target in vaccine development. Multiple functionally distinct helper CD4+ T cell subsets can arise in response to a single invading pathogen, complicating the identification of rare memory CD4+ T cells. Here we found that expression of Id3, an inhibitor of E protein transcription factors, identified a population of cells within both the CD4+ Tfh and Th1 helper lineages that exhibited memory potential in response to secondary infection. Notably, a subset of Th1 memory cells expressing Id3 exhibited enhanced expansion upon response to pathogen, generating both Th1 and Tfh secondary effector cell populations, and displayed enrichment of key molecules associated with memory potential when compared to Id3lo Th1 cells. Thus, Id3 expression serves as an important marker of multipotent memory CD4+ T cells.

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RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

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Genome-wide maps of chromatin state in naïve CD4+ T cells, WT/EZH2-null TFH and TH1 cells

Project description:ATAC-Seq experiments were performed to elucidate the chromatin state changes among naïve CD4+ T cells, WT follicular helper T (TFH) cells and WT type 1 helper T (TH1) cells Day2 (D2), Day5 (D5), Day8 (D8) post-LCMV-Armstrong infection, as well as EZH2-null (KO) TFH and TH1 at Day8 post-LCMV-infection. The analysis suggested stringent lineage-specific mode of chromatin accessibility in each group, indicating chromatin remodeling is tightly associated with TFH versus TH1 lineage differentiation in response to acute viral infection. Furthermore, the comparison between wild-type and EZH2-null TFH cells showed that less-opening state of certain chromatin accessible region in TFH-differentiation associated genes in the formers, suggesting EZH2 led to permissive chromatin accessibility primarily at specific regions of TFH-associated genes. H3K27me3-ChIP-seq was performed in WT TFH and TH1 cells to confirm the deposition of the histone marks at those loci.

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Expression data from virus-specific mouse Th1 and Tfh CD4 T cell subsets after LCMV infection

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