Project description:Genetically engineered mouse glioblastoma tumors were flow cytometry sorted into CD45 positive and CD45 negative populations followed by single cell RNA seq.
Project description:Genetically engineered mouse glioblastoma tumors and normal cells at different stages were flow cytometry sorted into CD45 positive and CD45 negative populations followed by single cell RNA seq.
Project description:Transcriptional profile of either RANK+/+ or RANK-/- MMTV-PyMT late carcinomas, orthotopically injected in syngeneic RANK+/+ mice. Once host mice developed tumors, CD45- tumor cells were sorted by flow cytometry in order to compare changes on tumor gene expression caused by the absence of RANK protein.
Project description:Exploration of proteome differences between CD45+ and CD45- cell types in renal cell carcinoma tumors and normal adjacent tissue patient samples.
Project description:We inflicted full thickness excisional wounds in the back skin of C57BL/6 wild-type mice. Injury-associated macrophages were isolated from wound tissue during the early inflammatory phase (4 days post injury) and in the late, scar forming phase (14 days post injury), sorted by flow cytometry (CD45+CD11b+F4/80+ wound cells), and subjected to RNAsequencing.
Project description:Transcriptional profiling of mouse dural CD45+ cells in comparing AAV-Controls with AAV-VEGFC treaded mice. Objective was to determine the effect of the VEGFC treatment on global gene expression of meningeal immune cells.