Project description:We performed miRNA microarray analysis to find differentially expressed miRNAs between nasopharyngeal carcinoma patients and normal control subjects In this study, 20 nasopharyngeal carcinoma patients and 10 normal control subjects were selected to collect plasma samples and perform miRNA microarray profiling
Project description:We performed miRNA microarray analysis to find differentially expressed miRNAs between nasopharyngeal carcinoma patients and normal control subjects
Project description:Microarray and quantitative real-time PCR (qRT-PCR) results showed that the Caspase-1 expression was increased while miR-513c-5p level was decreased substantially in DVT patients, and there was significant negative correlation between miR-513c-5p and Caspase-1. Knockdown of miR-513c-5p could aggravate DVT formation by enhancing Caspase-1-mediated pyroptosis, while overexpression of miR-513c-5p could alleviate DVT formation via inhibiting Caspase-1 expression. we identified pyroptosis of PBMCs with elevated expression of Caspase-1 in DVT patients. miRNA microarray analysis revealed miR-513c-5p as the most significantly down-regulated miRNA in DVT and the receiver operating characteristic (ROC) analysis showed that miR-513c-5p level could sensitively discriminate DVT from healthy controls.
Project description:In the present study through TLDA analysis we looked into the miRNA differential expression in peripheral blood of PCOS patients v/s control women. The results implicated that many signalling networks as MAPK pathway, Androgen signaling, Insulin signaling and Immune signaling are regulated in peripheral blood of PCOS patients. The data indicate that there is generic PCOS specific gene expression in peripheral blood of PCOS subjects which can reflect the same from other PCO tissues. Total RNA was extracted from peripheral blood of 4 PCOS patients and 4 control subjects and compared for miRNA diferential expression through TLDA
Project description:To investigated dysregulated miRNAs in human MDS patients, we performed miRNA-sequencing (miRNA-seq) of serum EVs from 38 MDS patients and 8 healthy subjects. The miRNA profile in EVs from MDS patients was distinctly clustered from that in healthy individuals. In addition, the miRNAs significantly upregulated in the MDS group target pathways linked to cell survival, proliferation, and MSC differentiation, indicating that they have remarkably similar properties to miRNAs in murine EVs from MDS cells. These results suggest that miRNAs play an essential role in the MSC impairment observed in MDS.
Project description:Comparative profiling of miRNA content within CD31+EVs comparing Ctrl and T2DM patients (5 vs 5 samples with each sample prepared from the pooled plasma of 4 subjects).