Project description:In recent years, the scale culture of Chinese soft-shelled turtle has developed rapidly. However, diseases in aquaculture are the main problems affecting the rapid and healthy cultivation. Strengthening the immunity of Chinese soft-shelled turtles is extremely important to control the infection of pathogenic bacteria. Bacillus has attracted attention as a probiotic supplement in aquatic feeds.In our previous studies, we found that the addition of Bacillus subtilis B10 to diets could increase survival rate, daily weight gain (DG) and feed conversion ratio (FCR) of Chinese soft-shelled turtles, improving the activities of digestive enzyme and optimizing the microbial communities of intestinal in Chinese soft-shelled turtle.However, the study on the mechanism of Bacillus subtilis B10 in Chinese soft-shelled turtle culture remains rare. Therefore, in this study, we used Bacillus subtilis B10 to feed the turtle, and used RNA-seq to explore its mechanism.
2022-09-01 | GSE183769 | GEO
Project description:RNA methylaton of chinese soft-shell turtle
Project description:To investigate the cellular responses of the Chinese soft-shelled turtle after niclosamide exposure, a total of 45 healthy juvenile turtles randomly selected and divided into three groups containing one control group (without NIC exposure, CG) and two experimental groups (exposed to 10 and 50 μg/L of NIC, respectively).
2024-01-01 | GSE225631 | GEO
Project description:Large intestine transcriptome of Chinese soft-shelled turtle
| PRJNA895971 | ENA
Project description:Analysis of intestinal microbiota in Chinese soft-shelled turtle
| PRJNA893835 | ENA
Project description:Transcriptome analysis on the liver of Chinese Soft-shelled Turtle (Pelodicus sinensis)
| PRJNA627320 | ENA
Project description:Liver Transcriptome Analysis Reveals the Hibernation Challenge in the Chinese Soft-Shelled Turtle
Project description:In the present study, the eggs of Chinese pond turtles (Mauremys reevesii) were incubated at three temperatures (26℃, 29℃ and 32℃). During the thermosensitive period (TSP) of incubation, the adrenal-kidney-gonad (AKG) complexes were sampled, and the transcriptome differences of the AKG samples were investigated using next-generation sequencing, then candidate genes associated with temperature-dependent sex determination of Reeves' Turtle were identified.
Project description:The Arabidopsis Tandem Zinc Finger 9 (TZF9) is a putative RNA-binding protein that is localized to Processing Body-like structures. A tzf9 knock out line showed attenuated response to the Pattern-Associated Molecular Pattern (PAMP), flg22 (doi: 10.1093/pcp/pct175). To uncover the molecular mechanism for this phenotype, global gene expression analysis covering total RNA (transcriptome) and polysome-associated mRNAs (translatome) was performed. For the latter, a transgenic line expressing a FLAG-tagged ribosomal protein RPL18 was crossed to the tzf9 mutant. The results show a strong 'uncoupling' between transcriptome and translatome in the mutant, indicating that TZF9 could be involved in global post-transcriptional regulation process.
