Insights into ROS-dependent signalling underlying transcriptomic plant responses to the herbicide 2,4-D
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PROVIDER: PRJNA743092 | ENA |
REPOSITORIES: ENA
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Project description:The synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) functions as an agronomic weed control herbicide. High concentrations of 2,4-D induce plant growth defects, particularly leaf epinasty and stem curvature. Although the 2,4-D phenotype is associated with ROS production, little is known about ROS-dependent signalling. In this study, by using T-DNA Arabidopsis mutants to silence peroxisomal acyl CoA oxidase 1 (acx1-2), we identified ACX1 as one of the main sources of ROS production and as partly the cause of the epinasty phenotype following the application of 2,4-D. Transcriptomic analyses of WT plants after treatment with 2,4-D revealed a ROS-related peroxisomal footprint in early plant responses, while other organelles, such as mitochondria and chloroplasts, are involved in later responses. Interestingly, a group of ACX1-dependent transcripts in plant responses to 2,4-D, previously associated with epinasty, is related to auxin biosynthesis, metabolism and signalling. We found that protein AUXIN SIGNALING F-BOX 3 (AFB3), a component of SCF (ASK-cullin-F-box) E3 ubiquitin ligase complexes, which functions as an auxin receptor and mediates Aux/IAA proteasomal degradation, acts downstream of ACX1 and is involved in the epinasty phenotype induced by 2,4-D. We also found that protein degradation associated with ubiquitin E3-RING and E3-SCF-FBOX in ACX1-dependent signalling in plant responses to 2,4-D is significantly regulated over longer treatment periods.
2021-12-01 | GSE179303 | GEO
Project description:Resistance to the phenoxy synthetic auxin herbicide 2,4-D is conferred by a deletion mutation in the degron tail of IAA2. An RNA-Seq experiment was used to assemble a reference transcriptome for the weed species Sisymbrium orientale, to quantify gene expression, and to identify sequence variants in the alignments. A 27 bp deletion was identified in the IAA2 gene. Forward genetics supported the role of this deletion mutation in resistance. The IAA2 deletion allele was transformed into Arabidopsis thaliana to confirm that it conferred 2,4-D resistance.
2020-10-08 | GSE159202 | GEO
Project description:This experiment was annotated by TAIR (http://www.arabidopsis.org). In this experiment we examined whole genome response to herbicidal levels of 2,4-D application. Arabidopsis plants (14 days) grown in vitro on MS medium in petri dishes were flooded with 1 mL stock of 2,4-D to enable root uptake. Plants were treated with 1.0 mM 2,4-D for a period of 1 hour immediately after which RNA was extracted and used for the microarray based experiments. the goal of this study was to explore the herbicidal mode of action of auxinic herbicide 2,4-D. Experimenter name = Chitra Raghavan Experimenter department = Trevor Stevenson Laboratory Experimenter institute = IRRI Experimenter address = Entomology and Plant Pathology Division IRRI Experimenter zip/postal_code = Manila 4031 Experimenter country = Philippines Keywords: compound_treatment_design
2007-01-08 | GSE5743 | GEO
Project description:This experiment was annotated by TAIR (http://www.arabidopsis.org). In this experiment we examined whole genome response to herbicidal levels of 2,4-D application. Arabidopsis plants (14 days) grown in vitro on MS medium in petri dishes were flooded with 1 mL stock of 2,4-D to enable root uptake. Plants were treated with 1.0 mM 2,4-D for a period of 1 hour immediately after which RNA was extracted and used for the microarray based experiments. the goal of this study was to explore the herbicidal mode of action of auxinic herbicide 2,4-D. Experimenter name = Chitra Raghavan; Experimenter department = Trevor Stevenson Laboratory; Experimenter institute = IRRI; Experimenter address = Entomology and Plant Pathology Division IRRI; Experimenter zip/postal_code = Manila 4031; Experimenter country = Philippines Experiment Overall Design: 3 samples were used in this experiment
2008-06-14 | E-GEOD-5743 | biostudies-arrayexpress
Project description:Background: The auxin herbicides 2,4-D and dicamba are commonly used for management of horseweed (Erigeron canadensis L, (syn: Conyza canadensis L)). Halauxifen-methyl is a new auxin herbicide and recently commercialized to control several broadleaf weed species under a range of sites and environmental conditions. While synthetic auxin herbicides have been used for over 70 years, the precise mode of action that leads to plant death has yet to be clearly characterized. As new chemical families are discovered and the use of these herbicides continues to increase, it is imperative to understand how synthetic auxin active ingredients work within the plant to cause an herbicidal effect. Results: At 1 hour after treatment (HAT), 48 genes were consistently upregulated across the three herbicides, many of which are involved in the auxin-activated signaling pathway and response to auxin. At 6 HAT, 735 genes were upregulated by all herbicide treatments including genes associated with hormone signaling, metabolism, transport, senescence, and gene expression. The GO terms representing the 501 genes downregulated in all herbicide treatments were broadly categorized under two major groups: ATP and photosynthesis. At 6 HAT, over 50% of the genes differential expressed among the three herbicide treatments were unique to a single active ingredient. Conclusion: This research presents a first look into the differential gene expression profiles in horseweed following a foliar application of synthetic auxin compounds that represent three unique chemical families. While there are an abundance of transcriptome similarities induced by each herbicide that accounts for the general auxin herbicide response, distinct gene expression changes exclusive to each compound cannot be ignored as a contributor to the mode of action.
2020-02-03 | GSE116958 | GEO
Project description:We used RNAseq to quantify trascript expression from three populations of Conyza sumatrensis before and 5 hours after treatment with 2,4-D. This study investigates different responses between a 2,4-D resistant biotype compared to a 2,4-D sensitive biotype.
2023-06-30 | GSE226337 | GEO
Project description:affy_sunflower_2010_13 - affy_sunflower_2010_13 - It concerns the interaction between ROS and hormones in dormancy release in sunflower seeds. ABA is responsible for dormancy maintenance, while GA and ethylene promote seed germination. Based on our results, ROS could represent good candidate to shift from a hormone signalling to another determining the dormancy state in sunflower seeds.-We aim to understand the mechanisms controlling sunflower seed dormancy at the transcriptomic level, by the application of treatments which maintain dormancy as ABA, or alleviate dormancy as ROS and ethylene. Transcripts comparison will be performed between dormant and non-dormant sunflower embryo imbibed 24h on water, on ABA, on methylviologen, a pro-oxidant compound or on ethylene. 12 arrays - SUNFLOWER; treated vs untreated comparison
2013-08-01 | E-GEOD-44165 | biostudies-arrayexpress
Project description:Resistance to the herbicide 2,4-D in Sisymbrium orientale conferred by a deletion mutation in the degron tail of IAA2
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Project description:Pseudomonas sp. 2,4-D Genome sequencing and assembly
| PRJNA990562 | ENA
Project description:This project aimed to investigate the effects of glyphosate-based herbicide Roundup LB Plus on bacteria. For this, ten environmental strains of Salmonella enterica were exposed to the increasing concentrations of Roundup over several passages to obtain Roundup-resistant mutants. Four stable re-sequenced resistant mutants and their respective ancestors were characterized by global proteomics in the presence and absence of sub-inhibitory (1/4xMIC) concentrations of the herbicide. By comparing the proteomes of the Roundup-challenged ancestors with constitutive non-challenged ancestors, it became possible to deepen the understanding of how Roundup stress affects naïve bacteria. Similarly, comparing Mutants versus Ancestors in the absence of Roundup allowed to understand how Roundup resistance constitutively affects bacterial physiology, while the comparison of Roundup-challenged mutants versus constitutive mutants helped improve the understanding of the inducible responses in the resistant background.
2020-09-22 | PXD019463 | Pride