Project description:The expression profile of HUVEC in response to IgG or HECD-1 treated exosomes We used data from this array to identify differentiatly expressed genes.
Project description:YAP knockdown in HUVEC elicits proliferation and cell cycle preogression defects. YAP deficient cells caused arrest in G1 and defects in S-phase entry. The microarray analysis was conducted to identify potential YAP targets that are involved in HUVEC cell cycle regulation
Project description:To assess if HUVEC and HUAEC differ in suscptibility to inflammation and inflammation resolution, the cells were stimulated for 24 hrs with TNF followed by RNA isolation. Two additional groups were first stimulated with TNF (24 hr) followed by TNF removal (12 and 24 hrs). Expression profiles were compared to basal conditions, i.e. cells cultured in normal medium We used micro-arrays to assess if Gene-xpression profiles between HUVEC and HUAEC differ under inflammatory conditions and under conditions of inflammation resolution
Project description:In this project, we aimed to explore the role of extracellular vesicles (EVs) in Zika virus (ZIKV) infection. We preferred antibody capture to ltracentrifugation for exosome isolation. The EVs derived from naive HUVEc cells or ZIKV-infected HUVEc cells were harvested and analyzed by mass spectrometry. The protein profile data understand the relationship between the ZIKV and EVs.
Project description:Human umbilical vein endothelial cells (HUVEC) were cultured in serum-free medium with 50 μmol/L ADMA (ADMA group) or without ADMA (NA group ). Asymmetric dimethylarginine is a typical uremic toxin which used to induce HUVEC injury.
Project description:Question Addresses: What is the gene expression profile from human umbilical vein endothelial cells (HUVEC) and human Jurkat T cells after irradiation (IR)? What, if any, is the effect of co-culturing these two cell types on gene expression? There are eight experimental conditions for this experiment: (1) non-irradiated HUVEC; (2) irradiated HUVEC; (3) non-irradiated Jurkat; (4) irradiated Jurkat; (5) non-irradiated HUVEC + non-irradiated Jurkat+; (6) non-irradiated HUVEC + irradiated Jurkat; (7) irradiated HUVEC + non-irradiated Jurkat; (8) irradiated HUVEC + irradiated Jurkat. A common, pooled reference consisting of RNA taken from conditions 1-8 as described above was used for all hybridizations.
Project description:HUVEC (N=3 isolates) were separately grown to sub-confluency, and to confluency. In order to study the effects of growth and contact inhibition on the transcriptome, the microarray gene expression profiles of these sub-confluent and confluent HUVEC were compared. Keywords: HUVEC, confluency, contact inhibition
