Project description:RNA Pull-Down-LC/MS screens interactive proteins with tRF-1-S25 in HUVEC, followed by aquiring the proteins . We Utilized tRF-1-S25 Pull-Down from HUVEC to acquire the interactive proteins. Then we used LC/MS to detect and analyze these interactive proteins with a label free method. Our goal is to distinguish whether existed interactive proteins in HUVEC. 480_FPEP210002780-1A.raw, 480_FPEP210002781-1A.raw, and 480_FPEP210002782-1A.raw are three replicates from HUVEC as control samples. 480_FPEP210002783-1A.raw, 480_FPEP210002784-1A.raw, and 480_FPEP210002785-1A.raw are three replicates from HUVEC as tRF-1-S25 samples.
Project description:To assess the transcriptome of HUVEC where the SENCR is knocked down. SENCR is a lncRNA we show stabilizes the endothelial cell adherens junction by binding CKAP4 and indirectly localizing CDH5 at the cell membrane. The RNA-seq study was carried out to ascertain whether the effects of SENCR could be attributed to a function associated with sponging endogenous microRNAs that target proteins of the adgherens junction.
Project description:Question Addresses: What is the gene expression profile from human umbilical vein endothelial cells (HUVEC) and human Jurkat T cells after irradiation (IR)? What, if any, is the effect of co-culturing these two cell types on gene expression? There are eight experimental conditions for this experiment: (1) non-irradiated HUVEC; (2) irradiated HUVEC; (3) non-irradiated Jurkat; (4) irradiated Jurkat; (5) non-irradiated HUVEC + non-irradiated Jurkat+; (6) non-irradiated HUVEC + irradiated Jurkat; (7) irradiated HUVEC + non-irradiated Jurkat; (8) irradiated HUVEC + irradiated Jurkat. A common, pooled reference consisting of RNA taken from conditions 1-8 as described above was used for all hybridizations.
Project description:We analyzed genome-wide transcriptional changes induced by ABTAA+Ang2, using RNA-seq analysis of HUVECs Examination of 3 different antibodies treated to HUVEC to analyze the effect of Tie2 activation
Project description:Purpose: To understand molecular and cellular effects of secreted sFRP2 to endothelial cells Methods: We performed RNA sequencing of 9 samples from HUVEC cultured in WT, sFRP2OE, LFS conditioned media for 16 hours Conclusion: apoptosis and angiogenetic molecular factors are activated in HUVEC by secreted sFRP2
