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Two auxinic herbicides alter Brassica napus changes in transcription in a time dependent manner

ABSTRACT: Two auxinic herbicides alter Brassica napus changes in transcription in a time dependent manner

PROVIDER: PRJNA744527 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15064644_1.fastq.gz	Fastqsanger.gz
	SRR15064644_2.fastq.gz	Fastqsanger.gz
	SRR15064645_1.fastq.gz	Fastqsanger.gz
	SRR15064645_2.fastq.gz	Fastqsanger.gz
	SRR15064646_1.fastq.gz	Fastqsanger.gz

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Two auxinic herbicides alter Brassica napus changes in transcription in a time dependent manner

Project description:The aim of this study was to investigate the molecular background by using different growth conditions under which three different auxinic herbicides were administered. These were halauxifen-methyl, and picloram at thre different harvest points after treatments (2, 48, 96 hours). Hal treatment lead to the upregulation of translational processes after 2 h, whereas auxin and hormone responses were upregulated at 48h and 96 h. Photosynthesis and mitochondrial pro-cesses were downregulated through all time points. Further, after 48 h translation was downreg-ulated. Pic treatment induced the hormone / auxin response already after 2 h, and this continued for the other time points. At 2 h and 48 h after treatment the translational processes were down-regulated and photosynthesis was downregulated after 48 h and 96 h. The more detailed analy-sis of the auxin response in the datasets indicate a role for GH3 genes, genes encoding auxin efflux proteins and confirm the upregulation of the ethylene pathway.

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Project description:High temperature stress results in yield loss and alterations to seed composition during seed filling in oilseed rape (Brassica napus). However, the mechanism underlying this heat response is poorly understood. In this study, we employed a microarray analysis with silique walls and seeds from the developing siliques (20 days after flowering) of Brassica napus that had undergone heat stress.

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Gene expression profiles during seed development and maturation in Brassica napus

Project description:Time course of gene expression profiles during seed development and maturation in Brassica napus were studied using Combimatrix Brassica microarray.

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Microarray expression analysis of the main inflorescence in B. napus

Project description:The effect of the number of pods on the main inflorescence (NPMIs) on seed yield in Brassica napus plants grown at high density is a topic of great economic and scientific interest. We sought to identify patterns of gene expression that are associated with inflorescence and PMI differentiation and development in Brassica napus.

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Gene expression profiles during seed development and maturation in Brassica napus

Project description:Time course of gene expression profiles during seed development and maturation in Brassica napus were studied using Combimatrix Brassica microarray. The time course expression of 90K Brassica napus EST contigs were measured at 8 developing seed stages of 10, 15, 20, 25, 30, 35, 40 and 45 DAF (days after flowering) using single color microarray

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Project description:MicroRNAs and siRNAs are important regulators of plant development and seed formation, yet their population and abundance in the oil crop Brassica napus are still less understood, especially at different developmental stages and among cultivars with varied seed oil contents. Here, we systematically analyzed the small RNA expression profiles of Brassica napus seeds at early embryonic developmental stages in a high oil content and a low oil content Brassica napus cultivars, both cultured in two environments. A total of 50 conserved miRNAs and 11 new miRNAs were identified, together with some new miRNA targets. Expression analysis revealed some miRNAs with varied expression levels in different seed oil content cultivars or at different embryonic developmental stages. A large amount of 23-nt small RNAs with specific nucleotide composition preference were also identified, which may present new classes of functional small RNAs.

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