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Targeted protein degradation reveals Pol II heterogeneity and functional diversity [PRO-Seq]

ABSTRACT: Targeted protein degradation reveals Pol II heterogeneity and functional diversity [PRO-Seq]

PROVIDER: PRJNA745447 | ENA |

REPOSITORIES: ENA

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Targeted protein degradation reveals Pol II heterogeneity and functional diversity [PRO-Seq]

Project description:RNA polymerase II (Pol II) subunits are thought to be involved in various transcription-associated processes, but it is unclear whether they play different regulatory roles in modulating gene expression. Here, we performed nascent and mature transcript sequencing after the acute degradation of 12 mammalian Pol II subunits and profiled their genomic binding sites and protein interactomes to dissect their molecular functions. We found that Pol II subunits contribute differently to Pol II cellular localization and transcription process and preferentially regulate RNA processing (such as RNA splicing and 3’ end maturation). Genes sensitive to the depletion of different Pol II subunits tend to be involved in diverse biological functions and show different RNA half-lives. Sequences, associated protein factors, and RNA structures are correlated with Pol II subunit-mediated differential gene expression. These findings collectively suggest that the heterogeneity of Pol II and different genes appear to depend on some of the subunits.

2022-08-09 | GSE179875 | GEO

Targeted protein degradation reveals Pol II heterogeneity and functional diversity

2022-08-09 | PXD035866 |

Targeted protein degradation reveals Pol II heterogeneity and functional diversity

Project description:Targeted protein degradation reveals Pol II heterogeneity and functional diversity

| PRJNA745987 | ENA

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChIP-Seq]

2022-08-09 | GSE179965 | GEO

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChAR-Seq]

2022-08-09 | GSE179962 | GEO

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [RNA-Seq]

2022-08-09 | GSE179873 | GEO

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChAR-Seq]

Project description:Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChAR-Seq]

| PRJNA745983 | ENA

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChIP-Seq]

Project description:Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChIP-Seq]

| PRJNA746071 | ENA

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [RNA-Seq]

Project description:Targeted protein degradation reveals Pol II heterogeneity and functional diversity [RNA-Seq]

| PRJNA745445 | ENA

Chromatin state changes induced by targeted degradation of MLL-AF9 are phenocopied by combined DOT1L and MENIN inhibition [PRO-seq]

Project description:Using PRO-seq we examined nascent transcriptional changes and RNA Pol II pausing following MLL-AF9 degradation in MLL-AF9-HA-FKBP12 transformed hCD34+ cells (HCB1) after 15, 30, and 180 minutes

2022-03-03 | GSE189105 | GEO

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