Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Targeted protein degradation reveals Pol II heterogeneity and functional diversity

ABSTRACT: Targeted protein degradation reveals Pol II heterogeneity and functional diversity

PROVIDER: PRJNA745987 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15097403_1.fastq.gz	Fastqsanger.gz
	SRR15097403_2.fastq.gz	Fastqsanger.gz
	SRR15097404_1.fastq.gz	Fastqsanger.gz
	SRR15097404_2.fastq.gz	Fastqsanger.gz
	SRR15097405_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 456

Similar Datasets

Targeted protein degradation reveals Pol II heterogeneity and functional diversity

Project description:RNA polymerase II (Pol II) subunits are thought to be involved in various transcription-associated processes, but it is unclear whether they play different regulatory roles in modulating gene expression. Here, we performed nascent and mature transcript sequencing after the acute degradation of 12 mammalian Pol II subunits and profiled their genomic binding sites and protein interactomes to dissect their molecular functions. We found that Pol II subunits contribute differently to Pol II cellular localization and transcription process and preferentially regulate RNA processing (such as RNA splicing and 3’ end maturation). Genes sensitive to the depletion of different Pol II subunits tend to be involved in diverse biological functions and show different RNA half-lives. Sequences, associated protein factors, and RNA structures are correlated with Pol II subunit-mediated differential gene expression. These findings collectively suggest that the heterogeneity of Pol II and different genes appear to depend on some of the subunits.

2022-08-09 | PXD035866 |

Targeted protein degradation reveals Pol II heterogeneity and functional diversity [ChIP-Seq]

2022-08-09 | GSE179965 | GEO