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A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

ABSTRACT: A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

PROVIDER: PRJNA745680 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15100966_1.fastq.gz	Fastqsanger.gz
	SRR15100966_2.fastq.gz	Fastqsanger.gz

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A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

Project description:A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

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A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

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A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:We used RNA-seq to detect genome-wide gene expression change when a reporter RNA was targeted by Cas13

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Project description:The CRISPR/Cas13 system has garnered attention as a potential tool for RNA editing. However, the degree of collateral activity among various Cas13 orthologs and their cytotoxic effects in mammalian cells remain contentious, potentially impacting their applications. In this study, we observed differential collateral activities for LwaCas13a and RfxCas13d in 293T and U87 cells by applying both sensitive dual-fluorescence (mRuby/GFP) reporter and quantifiable dual-luciferase (Fluc/Rluc) reporter, with LwaCas13a displaying notable activity contrary to previous reports. However, significant collateral RNA cleavage exerted only a modest impact on cell viability. Furthermore, the collateral activity of LwaCas13a mildly impeded but did not arrest, porcine embryo development. Our findings reveal that distinct collateral RNA cleavage by Cas13 slightly suppresses mammalian cell proliferation and embryo development. This could account for the lack of reported collateral effects in numerous prior studies and offers new insights into the implications of the collateral activity of Cas13 for clinical application.

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