Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

ABSTRACT: The study investigates how varying target expression levels influences self-targeting and defense using Cas13a. We see how immunity is activated only over a target transcription threshold, which is variable between targets. A gRNA library targeting transcripts from the whole coding genome of E. coli MG1655 confirms the importance of the transcription threshold in determining immunity and shows that most of the cellular transcripts do not induce cytotoxicity.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE179914 | GEO | 2022/06/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [Genome wide screen]

Project description:The study investigates how varying target expression levels influences self-targeting and defense using Cas13a. We see how immunity is activated only over a target transcription threshold, which is variable between targets. A gRNA library targeting transcripts from the whole coding genome of E. coli MG1655 confirms the importance of the transcription threshold in determining immunity and shows that most of the cellular transcripts do not induce cytotoxicity.

2022-06-08 | GSE179913 | GEO

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-06-08 | GSE179915 | GEO

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

Project description:A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

| PRJNA745581 | ENA

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

Project description:A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [RNA-seq]

| PRJNA745680 | ENA

A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [Genome wide screen]

Project description:A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [Genome wide screen]

| PRJNA745681 | ENA

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [Screen]

Project description:CRISPR-Cas13 systems have been adapted as versatile toolkits for RNA-related applications. Here we systematically evaluate the performance of several prominent Cas13 family effectors (Cas13a, Cas13b and Cas13d) under lentiviral vectors and reveal surprisingly differential defects and characteristics of these systems. Using RNA immunoprecipitation sequencing, transcriptome profiling, biochemistry analysis and high-throughput CRISPR-Cas13 screening approaches, we determine that each Cas13 system has its intrinsic RNA targets in mammalian cells. Viral process-related host genes can be targeted by Cas13 and affect the production of fertile lentiviral particles, thereby restricting the utility of lentiviral Cas13 systems. Multiple RNase activities of Cas13 are involved in endogenous RNA targeting. Unlike target-induced collateral effect, intrinsic RNA targeting can be specific, target-independent and dynamically tuned by varied states of Cas13 nucleases. Our work not only provides guidance to appropriately utilize lentiviral Cas13 systems, but also raises cautions about intrinsic RNA targeting during Cas13-based basic and therapeutic applications.

2023-10-09 | GSE202841 | GEO

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [RIP-seq]

2023-10-09 | GSE202898 | GEO

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [RNA-seq]

2023-10-09 | GSE202838 | GEO

Project description:RNA targeting with CRISPR-Cas13a

| PRJNA383832 | ENA

Homo sapiens

Project description:Profiling Cas13a activity targeting influenza virus

| PRJNA476504 | ENA