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CHIP-seq analysis about H3K9me3 change after Zscan4 overexpression

ABSTRACT: CHIP-seq analysis about H3K9me3 change after Zscan4 overexpression

PROVIDER: PRJNA746248 | ENA |

REPOSITORIES: ENA

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CHIP-seq analysis about H3K9me3 change after Zscan4 overexpression

Project description:Transcription factor Zscan4 was newly discovered to be a marker of the intermediate state of 2-cell like cells . In addition, WGCNA discovered a strong positive correlation between the expression of Zscan4 and that of MT2/MERVL. Hence, we examined the possibility for Zscan4 as an activator of MT2/MERVL.

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Project description:A small subgroup of embryonic stem cells (ESCs) exhibit molecular features similar to those of two-cell embryos (2C). Multiple chromatin modifiers associated with H3K9 trimethylation (H3K9me3) have been implicated in the mediation of the 2C-like transition. However, it remains elusive how H3K9me3 is remodeled and its association with totipotent cells. Here, we analyze the genome-wide change in H3K9me3 in 2C-like transitions. We revealed that a large fraction of trophoblast-stem-cell specific enhancers undergoes loss of H3K9me3 exclusively in MERVL+/Zscan4+ cells during 2C-like transitions.

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Genome-wide maps of H3K9me3 in control ESCs and Rif1 knockdown ESC.

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ChIP-chip of H3K9me3 in Drosophila at different time points of development

Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. This Series contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K9me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM382158..GSM382166]: ChIP-chip of H3K9me3 in Drosophila embryos at 12-16 hours of development [GSM384690..GSM384698]: ChIP-chip of H3K9me3 in Drosophila embryos at 0-4 hours of development [GSM384723..GSM384731]: ChIP-chip of H3K9me3 in Drosophila embryos at 4-8 hours of development [GSM384732..GSM384740]: ChIP-chip of H3K9me3 in Drosophila embryos at 16-20 hours of development [GSM384741..GSM384749]: ChIP-chip of H3K9me3 in Drosophila embryos at 20-24 hours of development [GSM384750..GSM384758]: ChIP-chip of H3K9me3 in Drosophila L1 larvae [GSM384760..GSM384768]: ChIP-chip of H3K9me3 in Drosophila L2 larvae [GSM418207..GSM418215]: ChIP-chip of H3K9me3 in Drosophila Adult male [GSM418216..GSM418224]: ChIP-chip of H3K9me3 in Drosophila embryos at 8-12 hours of development [GSM442371..GSM442379]: ChIP-chip of H3K9me3 in Drosophila Adult male - Set2 [GSM442380..GSM442388]: ChIP-chip of H3K9me3 in Drosophila Pupae [GSM442389..GSM442397]: ChIP-chip of H3K9me3 in Drosophila L3 larvae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each combination of time-point and antibody, triplicate ChIP experiments have been performed and hybridized on Agilent 244K arrays. 3 arrays per genome have been used so that each time-point is a set of 9 tiling arrays.

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