Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling of FaDu cells overexpressing TINCR-HA-FLAG

ABSTRACT: Gene expression profiling of FaDu cells overexpressing TINCR-HA-FLAG

PROVIDER: PRJNA747713 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15182308_1.fastq.gz	Fastqsanger.gz
	SRR15182308_2.fastq.gz	Fastqsanger.gz
	SRR15182309_1.fastq.gz	Fastqsanger.gz
	SRR15182309_2.fastq.gz	Fastqsanger.gz
	SRR15182310_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Gene expression profiling of FaDu cells overexpressing TINCR-HA-FLAG

Project description:Differential gene expression analysis of FaDu cells overexpressing TINCR-HA-FLAG or an empty vector.

2024-07-16 | GSE180301 | GEO

Absolute quantification analysis of ubiquitin chains attached to Nrf1 purified from NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2.

Project description:We examined ubiquitin-linkages on Nrf1 purified from cell lysates of NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2. The ubiquitin linkage types were quantified using the LC-MS/MS-based absolute quantification method, ubiquitin parallel reaction monitoring (Ub-AQUA/PRM).

2024-08-06 | PXD053753 | Pride

ChIP-chip data of HA-Yra1 and Rrm3-Flag in wild-type and cells overexpressing YRA1

Project description:Transcription is a major obstacle for replication fork progression and a cause of genome instability. Yra1 is an essential nuclear factor of the evolutionarily conserved family of hnRNP-like export factors that when overexpressed impairs mRNA export and cell growth. Through this ChIP-chip analysis it is shown that Yra1 binds to active chromatin and is enriched at telomeres when it is overexpressed, in agreement with a possible role of this mRNP factor in the maintenance of telomere integrity. Our data indicate that YRA1 overexpression correlates with replication impairment as inferred by the increase of Rrm3, a helicase involved in the replication fork progression, at transcribed genes and telomeres. ChIP-chip studies were perfomed with antibodies against HA-tagged Yra1 protein in wild-type cells and cells overexpressing YRA1 of the yeast S. Cerevisiae, as well as Flag-tagged Rrm3 protein in both wild-type and cells overexpressing YRA1.

2016-03-11 | E-GEOD-68486 | biostudies-arrayexpress

RNAseq analysis of MM13 and MM2 metastatic melanoma PDX cells overexpressing TINCR

Project description:Purpose: Study the changes in cell transcriptome upon overexpression of lncRNA TINCR Methods: Primary melanoma cell line WM902B was transduced with lientiviruses expressing Scrambled and TINCR directed shRNA hairpins Results: we found that TINCR overexpression induces partial reversion of invasive phenotype

2020-11-25 | GSE125834 | GEO

Purification of endogenously FLAG-HA-tagged KAT6B from E14 ESCs

Project description:An N-terminal FLAG-HA tag was introduced into the endogenous Kat6b locus by CRISPR genome editing and purified by FLAG affinity purification using anti-FLAG agarose beads.

2025-01-02 | PXD015988 | Pride

Identification of proteins interacting with HA-FLAG-Eya1

Project description:To explore the spectrum of Eya1-interacting partner proteins, we thus generated a stable HEK293 cell line that constitutively express the 2HA–3FLAG-Eya1 (HF-Eya1) protein, purified HF-Eya1 from the cell extracts by a tandem affinity purification protocol to reduce background (first with anti-FLAG and then with anti-HA to purify FLAG-elute) and analyzed 12bands (B1-B12) using mass spectrometry.

2022-10-12 | PXD033955 | Pride

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) ) on the transcriptome of CD44-overexpressing IMR90 cells.

2020-04-25 | E-MTAB-8943 | biostudies-arrayexpress

Control of Somatic Tissue Differentiation by the Long Non-Coding RNA TINCR (HTS)

Project description:Several of the thousands of human long non-coding RNAs (lncRNAs) have been functionally characterized; however, potential roles for lncRNAs in somatic tissue differentiation remain poorly understood. Here we show that a 3.7kb lncRNA, terminal differentiation-induced ncRNA (TINCR), controls human epidermal differentiation by a post-transcriptional mechanism. TINCR is required for high mRNA abundance of key differentiation genes, many of which are mutated in human skin diseases, including FLG, LOR, ALOXE3, ALOX12B, ABCA12, CASP14 and ELOVL3. TINCR-deficient epidermis lacked terminal differentiation ultrastructure, including keratohyalin granules and intact lamellar bodies. Genome-scale RNA interactome analysis revealed that TINCR interacts with a suite of differentiation mRNAs. TINCR-mRNA interaction occurs through a 25 nucleotide M-bM-^@M-^\TINCR boxM-bM-^@M-^] motif which is strongly enriched in interacting mRNAs and required for TINCR binding. A high-throughput screen to analyze TINCR binding capacity to ~9,400 human recombinant proteins revealed direct binding of TINCR RNA to the Staufen1 (STAU1) protein. STAU1-deficient tissue recapitulated the impaired differentiation seen with TINCR depletion. Loss of UPF1 and UPF2, both of which are required for STAU1-mediated RNA decay (SMD), however, lacked differentiation impacts. Instead, the TINCR/STAU1 complex seems to mediate stabilization of differentiation mRNAs, such as KRT80. These data identify TINCR as a key lncRNA required for somatic tissue differentiation, which occurs through inducible lncRNA binding to differentiation mRNAs to ensure their expression. Examination of TINCR-binding RNAs using two independent pools of TINCR-targeting oligos

2012-10-10 | E-GEOD-40121 | biostudies-arrayexpress

RNAseq analysis of MM13 and MM2 metastatic melanoma PDX cells overexpressing TINCR

Project description:RNAseq analysis of MM13 and MM2 metastatic melanoma PDX cells overexpressing TINCR

| PRJNA517676 | ENA

Identification of small RNAs interacting with FLAG/HA(FH)-tagged LARP7

Project description:Identification of small RNAs interacting with FLAG/HA(FH)-tagged LARP7

| PRJNA522460 | ENA