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Rhizobium leguminosarum strain:3841 | cultivar:viciae

ABSTRACT: Transcriptome of Rhizobium leguminosarum 3841 during colonization of pea rhizosphere

PROVIDER: PRJNA748006 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15193264.fastq.gz	Fastqsanger.gz

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Isolation and characterisation of a 14kDa adhesin protein from Rhizobium leguminosarum biovar viciae 3841

Project description:In this project we aimed to identify a 14 kDa adhesin protein from Rhizobium leguminosarum biovar viciae 3841 unsing in gel digestion coupled to LC-MS/MS.

2024-08-19 | PXD029089 | Pride

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto pea seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.

2008-11-30 | E-MEXP-1925 | biostudies-arrayexpress

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 grown on acetate versus glucose

Project description:Rhizobium leguminosarum biovar viciae strain 3841 was grown on glucose ammonia AMS versus acetate ammonia AMS and gene expression compared

2009-06-11 | E-MEXP-1963 | biostudies-arrayexpress

Transcription profiling of bacteroid Rhizobium leguminosarum strain 3841 wild type vs bacA mutant harvested from pea plant nodules

Project description:Wildtype bacteroids of Rhizobium leguminosarum biovar viciae 3841 were compared to a bacA mutant. In both cases bacteroids were harvested from 7 day old nodules from pea plants.

2009-01-06 | E-MEXP-1966 | biostudies-arrayexpress

Rhizobium leguminosarum bv. viciae strain:3841

Project description:Rhizobium leguminosarum bv. viciae strain:3841 Epigenomics

| PRJNA243966 | ENA

Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841 grown rhizosphere of either pea, alfalfa or sugarbeet

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 days in the rhizosphere of either 7 day old pea, alfalfa or sugarbeet before being harvested.

2010-08-03 | E-MEXP-2849 | biostudies-arrayexpress

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto vetch seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum biovar viciae strain 3841 was inoculated onto Viccia cracca seeds and bacteroids were harvested at 28d. Gene expression was then compared to free-living Rhizobium leguminosarum biovar viciae strain 3841 grown on succinate ammonia AMS.

2008-11-30 | E-MEXP-1924 | biostudies-arrayexpress

Antioxidant mechanism of glutaredoxins and their role in symbiotic nitrogen fixation in Rhizobium leguminosarum bv. viciae 3841

Project description:Glutaredoxins (Grx) are redoxin family proteins that reduce disulfifides and mixed disulfifides between glutathione and proteins. Rhizobium leguminosarum 3841 contains three genes coding for glutaredoxins: RL4289 (grxA) codes for a dithiolic glutaredoxin, RL2615 (grxB) codes for a monothiol glutaredoxin, while RL4261 (grxC) codes for a glutaredoxin-like NrdH protein. In this study, we have generated mutants which have interrupted one, two, or three glutaredoxin genes. These mutants had no different growth phenotypes from the wild type RL3841. Mutation of grxC did not affect R. leguminosarum antioxidant or symbiotic capacities, while grxA-derived or grxB-derived mutants decreased the antioxidant and fixation nitrogen capacity. grxA-derived mutants was severely impaired in there rhizosphere colonization, and formed maller nodules with defects of bacteroid differentiation. whereas nodules induced by grxB-derived mutants contained abnormally thick cortexs and prematurely senescent bacteroids. Surprisingly the grx triple mutant presented an enhanced defect in antioxidant and symbiotic capacities of R. leguminosarum. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential grx triple mutant root bacteroids in response to the wild type infection. 137 differentially expressed proteins were identified including 56 up-regulated and 81 down-regulated proteins. By sorting the identified proteins according to metabolic function, twenty-eight proteins were involved in transporter activity, twenty proteins were related to stress response and virulence, and sixteen proteins were related to amino acid metabolism. Overall, R. leguminosarum glutaredoxins play an important role in root nodule symbiosis by involving the functions of anti-oxidant defenses, Fe-S cluster assembly, and control of bacteroid differentiation and senescence.

2020-12-02 | PXD017835 | Pride

Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841grown in pea, alfalfa or sugarbeet rhizospheres compared to bacteria grown on glucose ammonia AMS medium

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 d in the pea or alfalfa or sugarbeet rhizosphere (plants all 7 d old at inoculation) and compared against bacteria grown on glucose ammonia AMS medium in the laboratory.

2010-08-04 | E-MEXP-2852 | biostudies-arrayexpress

Transcription profiling of Rhizobium leguminosarum biovar viciae grown on acetoacetate ammonia versus glucose ammonia

Project description:Rhizobium leguminosarum biovar viciae strain 3841 was grown on acetate ammonia AMS and glucose ammonia AMS and gene expression between the two cultures compared

2009-06-11 | E-MEXP-1964 | biostudies-arrayexpress

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