Project description:Purpose: This study seeks to determine the role of Hedgehog signaling in larynx development. Methods: To determine how Hedgehog signaling directs larynx epithelial morphogenesis we used genomic approaches to identify a novel set of Hedgehog target genes within the early larynx. To this end, we performed bulk RNA-seq on 3-pooled E10.5 (32-35S) larynx tissues from wild-type (2 replicates) and Shh-/- (2 replicates) embryos. From this experiment we identified 1873 differentially expressed genes (p-value <0.05). Results: Hedgehog is essential for maintaining the identity of foregut-derived epithelial cells within the larynx vocal folds. In the absence of HH signaling these epithelial cells undergo specific transcriptional changes to initiate a partial epithelial-to-mesenchymal transition, migrate out of the epithelial layer and under go cell death. The layer is then replaced by a different population of cells.

2022-11-30 | GSE190281 | GEO

Oral cavity

Project description:Oral cavity Targeted loci

| PRJNA622970 | ENA

Ectopleura larynx strain:wild

Project description:Ectopleura larynx strain:wild Transcriptome or Gene expression

| PRJNA215258 | ENA

oral cavity

Project description:oral cavity Raw sequence reads

| PRJNA953964 | ENA

Single cell transcriptome of the mouse larynx

Project description:Microbiota play a critical role in training and development of major components of host innate and adaptive immune system. We present the cellular landscape of the upper airway, specifically the larynx, by establishing a reference single-cell atlas and dissected the role of microbiota in laryngeal cell development and functions at a single-cell resolution by comparing the transcriptomes of conventionally raised and germ-free mouse larynges.

2023-08-01 | GSE203233 | GEO

Human oral cavity

Project description:Human oral cavity microbiota

| PRJNA918786 | ENA