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Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast ATAC-seq]

ABSTRACT: Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast ATAC-seq]

PROVIDER: PRJNA748593 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15206369_1.fastq.gz	Fastqsanger.gz
	SRR15206369_2.fastq.gz	Fastqsanger.gz
	SRR15206370_1.fastq.gz	Fastqsanger.gz
	SRR15206370_2.fastq.gz	Fastqsanger.gz
	SRR15206371_1.fastq.gz	Fastqsanger.gz

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Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast ATAC-seq]

Project description:Tbx3 regulates liver development in the mouse, but it's role in human liver development is unclear. We generated TBX3 knockout pluripotent stem cell lines and found that liver differentiation is also impaired in humans. Interestingly we noted expression of pancreas-specific genes during the liver differentiation in the TBX3 knockout line. To further investigate this phenomenon, we performed ATAC-seq on cells at the hepatoblast stage of the liver differentiation. These studies demonstrated that the loss of TBX3 enhances pancreatic-gene signature, implicating TBX3 as a regulator of fate decisions.

2021-10-14 | GSE180527 | GEO

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast RNA-seq]

Project description:Tbx3 regulates liver development in the mouse, but it's role in human liver development is unclear. We generated TBX3 knockout pluripotent stem cell lines and found that liver differentiation is also impaired in humans. Interestingly we noted expression of pancreas-specific genes during the liver differentiation in the TBX3 knockout line. To further investigate this phenomenon, we performed RNA-seq on cells at the hepatoblast stage of the liver differentiation. These studies demonstrated that the loss of TBX3 enhances pancreatic-gene signature, implicating TBX3 as a regulator of fate decisions.

2021-10-14 | GSE180526 | GEO

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast RNA-seq]

Project description:Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Hepatoblast RNA-seq]

| PRJNA748592 | ENA

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Pancreas RNA-seq]

Project description:Tbx3 regulates liver development in the mouse, but it's role in human liver development is unclear. We generated TBX3 knockout pluripotent stem cell lines and found that liver differentiation is also impaired in humans. Interestingly we noted expression of pancreas-specific genes during the liver differentiation in the TBX3 knockout line.To further investigate this phenomenon, we performed RNA-seq on cells from two stages of the pancreatic differentation. These studies demonstrated that the loss of TBX3 enhances pancreatic-gene signature, implicating TBX3 as a regulator of fate decisions.

2021-10-14 | GSE180525 | GEO

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells

Project description:Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells

| PRJNA748588 | ENA

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Pancreas RNA-seq]

Project description:Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Pancreas RNA-seq]

| PRJNA748594 | ENA

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells.

Project description:Tbx3 has been identified as a regulator of liver development in the mouse, but its function in human liver development remains unknown. TBX3 mutant human pluripotent stem cell (PSC) lines were generated using CRISPR/Cas9 genome editing. TBX3 loss led to impaired liver differentiation and an upregulation of pancreatic gene expression, including PDX1, during a hepatocyte differentiation protocol. Other pancreatic genes, including NEUROG3 and NKX2.2, displayed more open chromatin in the TBX3 mutant hepatoblasts. Using a pancreatic differentiation protocol, cells lacking TBX3 generated more pancreatic progenitors and had an enhanced pancreatic gene expression signature at the expense of hepatic gene expression. These data highlight a potential role of TBX3 in regulating hepatic and pancreatic domains during foregut patterning, with implications for enhancing the generation of pancreatic progenitors from PSCs.

| S-EPMC8580886 | biostudies-literature

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-14 | GSE180528 | GEO

Loss of MC1R signaling implicates TBX3 in pheomelanogenesis and melanoma predisposition

Project description:501mel cells engineered to express ectopically doxycyclin inducible TBX3 3xHA-tagged. Cells were seeded and treated with doxcyclin to achive expression comparable to endogeneous TBX3. 15x 15-cm dish worth of cells were collected for each replicate of ChIP-seq experiments.

2023-08-07 | GSE223256 | GEO

TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration

Project description:We investigated transcriptional profiles of Ptf1a-Cre driven homozygous Tbx3-KO and wildtype pancreata 72h and 168h after onset of acute pancreatitis.

2023-02-24 | GSE216889 | GEO

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