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Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of Raptor

ABSTRACT: Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of Raptor

PROVIDER: PRJNA749260 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15225343_1.fastq.gz	Fastqsanger.gz
	SRR15225343_2.fastq.gz	Fastqsanger.gz
	SRR15225344_1.fastq.gz	Fastqsanger.gz
	SRR15225344_2.fastq.gz	Fastqsanger.gz
	SRR15225345_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of Raptor

Project description:Recent studies have shown that mTOR signalling have a promotive effect on cancer. In our clinical work, we found that mTOR signalling is commonly activated in laryngeal cancer tissues and influence the metabolism of cancer cells. To investigate how mTORC1 exerts these functions and its downstream regulatory genes, we knocked down Raptor in the laryngeal cancer cell line LIU-LSC-1 and sequenced the transcriptome of wild-type and Raptor knockdown cells.

2022-11-03 | GSE180702 | GEO

Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of ITGA5

Project description:Recent studies have shown that integrins have a promotive effect on cancer. In our clinical work, we found that integrin subunit alpha 5 (ITGA5) was highly expressed in laryngeal cancer tissues. Further in vitro functional assays showed that ITGA5 promotes proliferation, migration and metastasis of laryngeal cancer cells. To investigate how ITGA5 exerts these functions and its downstream regulatory genes, we knocked down ITGA5 in the laryngeal cancer cell line LIU-LSC-1 and sequenced the transcriptome of wild-type and ITGA5 knockdown cells.

2022-11-03 | GSE180147 | GEO

Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of ITGA5

Project description:Transcriptomic analysis of human laryngeal carcinoma cell line LIU-LSC-1 with depletion of ITGA5

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Effect of PFKP deletion on gene expression in laryngeal carcinoma cells LIU-LSC-1

Project description:To investigate the role of PFKP in laryngea cancer, we established a LIU-LSC-1 cell line with PFKP knocked down by lentivirus technique. We then performed gene expression profiling targeting the RNA-seq data of the control and PFKP knockdown groups.

2024-08-08 | GSE248242 | GEO

Effect of PFKP deletion on gene expression in laryngeal carcinoma cells LIU-LSC-1

Project description:Effect of PFKP deletion on gene expression in laryngeal carcinoma cells LIU-LSC-1

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Campoletis raptor

Project description:Campoletis raptor overview

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Raptor/mTORC1-dependent metabolic programming of exit from quiescence underlies T cell activation and fate decisions

Project description:Naïve T cells respond to antigen stimulation by exiting from quiescence into clonal expansion and functional differentiation, but the control mechanism is elusive. Here we describe that Raptor/mTORC1-dependent metabolic reprogramming is a central determinant of this transitional process. Loss of Raptor abrogates T cell priming and Th2 cell differentiation, although Raptor function is less important for continuous proliferation of actively cycling cells. mTORC1 coordinates multiple metabolic programs in T cells including glycolysis, lipid synthesis and oxidative phosphorylation to mediate antigen-triggered exit from quiescence. mTORC1 further links glucose metabolism to the initiation of Th2 differentiation by orchestrating cytokine receptor expression and cytokine responsiveness. Activation of Raptor/mTORC1 integrates T cell receptor (TCR) and CD28 co-stimulatory signals in antigen-stimulated T cells. Our studies identify a Raptor/mTORC1-dependent pathway linking signal-dependent metabolic reprogramming to quiescence exit, and this in turn coordinates lymphocyte activation and fate decisions in adaptive immunity. We used microarrays to explore the gene expression profiles differentially expressed in CD4+ T-cells from wild-type (WT) and CD4(cre) x Raptor(fl/fl) mice before and after stimulation with anti CD3/CD28 antibodies.

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Gene-expression changes resulting from loss of the mTORC1 component Raptor in murine hematopoietic stem and progenitor cell-enriched populations (HSPC)

Project description:We investigated the role of mTORC1 in murine hematopoiesis by conditionally deleting the Raptor gene in murine hematopoietic stem cells. We observed mutliple alterations evoked by Raptor loss in hematopoiesis and profiled gene-expression alterations induced by raptor loss in Flt3-Lin-Sca1+cKit+ hematopoietic stem and progenitor enriched cell populations, 5 weeks post Raptor deletion. Flt3-Lin-Sca1+cKit+ cells were flow sorted from mice containing homozygous floxed alleles for exon 6 of the Raptor gene in the presence (MT group) or absence (WT group) of the MxCre transgene, which was induced with injections of mice with pIpC 5 weeks before cell isolation.

2012-09-19 | E-GEOD-32265 | biostudies-arrayexpress

Microarray Analysis Gene Expression Profiles in Laryngeal Muscle After Recurrent Laryngeal Nerve Injury

Project description:The pathophysiology of recurrent laryngeal nerve (RLN) transection injury is rare in that it is characteristically followed by a high degree of spontaneous reinnervation, with reinnervation of the laryngeal adductor complex (AC) preceding that of the abducting posterior cricoarytenoid (PCA) muscle. Here, we aim to elucidate the differentially expressed myogenic factors following RLN injury that may be at least partially responsible for the spontaneous reinnervation. F344 male rats underwent RLN injury or sham surgery (n=12). One week after RLN injury, larynges were harvested following euthanasia. mRNA was extracted from PCA and AC muscles bilaterally, and microarray analysis was performed using a full rat genome array. Microarray analysis of denervated AC and PCA muscles demonstrated dramatic differences in gene expression profiles, with 205 individual probes that were differentially expressed between the denervated AC and PCA muscles, and only 14 genes with similar expression patterns. The differential expression patterns of the AC and PCA suggest different mechanisms of reinnervation. The PCA showed the gene patterns of Wallerian degeneration, while the AC expressed the gene patterns of reinnervation by adjacent axonal sprouting. This finding may reveal important therapeutic targets applicable to RLN and other peripheral nerve injuries. Compare mRNA expression from injured tissue (recurrent laryngeal nerve transection injury) to normal tissue from two tissues laryngeal adductor complex and posterior cricoarytenoid muscles.

2016-07-01 | E-GEOD-72361 | biostudies-arrayexpress

Response to salt stress of Raptor mutants

Project description:ra12-03_raptor-nacl - Characterization to raptor mutants (partner of the kinase TOR) and of their answer to the salt stress. - Sowing on metal grids on MS/2 medium with continuous light. Transfer after 12 days of culture for 3h to liquid MS/2 medium containing or not 200 mM NaCl. 7 dye-swap - gene knock out, treated vs untreated comparison

2013-07-01 | E-GEOD-47391 | biostudies-arrayexpress

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