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CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in NHP

ABSTRACT: CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in NHP

PROVIDER: PRJNA749327 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15234578.fastq.gz	Fastqsanger.gz
	SRR15234579.fastq.gz	Fastqsanger.gz
	SRR15234580.fastq.gz	Fastqsanger.gz
	SRR15234581.fastq.gz	Fastqsanger.gz
	SRR15234582.fastq.gz	Fastqsanger.gz

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CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in NHP

Project description:The RV144 HIV vaccine trial remains the only study to demonstrate significant protection from future HIV-1 acquisition. One of the key components of the RV144 vaccine was the use of the canarypox vector ALVAC as the priming component. Since AIDSVAX, the booster component, alone failed to provide protection we hypothesized that the ALVAC prime contributed significantly to the generation of protection. To test this, we designed a NHP immunogenicity trial to mechanistically link ALVAC vaccination with the magnitude of V1V2 titers, the most significant immune correlate of reduced HIV-1 acquisition in RV144. Our objective was to use a systems biology approach to identify the transcription factors, target genes and immune pathways which were being induced by ALVAC vaccination and associated with higher V1V2 titers. We identified the transcription factor CREB1 and its target genes as rapidly induced by ALVAC in multiple immune subsets and that CREB1 drives the expression and activation of a network of other TFs which are critical for modulating immune responses. Pathways induced by this ALVAC-CREB1 axis include lymphocyte/leukocyte migration, lymphocyte differentiation, antigen processing and presentation, T cell co-stimulation and cytokine signaling.

2021-07-25 | GSE180748 | GEO

CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in Human PBMC

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Microarray analysis of correlates of vaccine immunogenecity following ALVAC vaccination in NHP in Study 36

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CHIP-Seq analysis of CREB1 binding after ALVAC vaccination

Project description:CHIP-Seq analysis of CREB1 binding after ALVAC vaccination

| PRJNA749324 | ENA

CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in Human PBMC

Project description:CHIP-Seq analysis of CREB1 binding after ALVAC vaccination in Human PBMC

| PRJNA749319 | ENA

Microarray analysis to identify optimal timepoint post-ALVAC vaccintation to investigate innate immune pathways

Project description:ALVAC-Comp was an NHP immunogenicity study designed to identify the temporal kinetics of innate immune activation by ALVAC-SIV vaccination. PAXGENE tubes were harvested at 16h, 24h, 48h and 72h post vaccination to investigate the speed, magnitude, and durability of ALVAC-SIV induced innate immune activation. This study was set up to identify the optimal timepoint(s) post-vaccination in which to probe the cellular and molecular signaling mechanisms of innate immunity induced by vaccination. Comparison of genes/pathways in common and unique to the 4 post-vaccination timepoints will reveal the order of innate immune induction post-vaccination.

2021-08-13 | GSE181996 | GEO

Microarray analysis of correlates of vaccine immunogenecity following ALVAC vaccination in NHP in Study 36

Project description:Microarray analysis of correlates of vaccine immunogenecity following ALVAC vaccination in NHP in Study 36

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CHIP-Seq analysis of CREB1 binding after ALVAC vaccination

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-25 | GSE180749 | GEO

Transcriptomic profiling of RV144 trial participants after vaccination with ALVAC-HIV and AIDSVAX B/E. [rv144]

Project description:The RV144 clinical trial evaluated the efficacy of a vaccine regimen that included ALVAC-HIV prime and AIDSVAX boost in preventing HIV-1 acquisition. The vaccine reduced the risk of HIV-1 acquisition by 31.2%; however the mechanisms that led to the protection induced by this vaccine remain poorly understood. Our objectives were to identify transcriptional correlates and mechanisms that could explain the reduced acquisition conferred by the vaccine. We assessed the transcriptomic profile of HIV Env stimulated peripheral blood mononuclear cells collected from 223 participants two weeks after vaccination and from 40 placebo recipients.

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Transcriptomic profiling of rhesus macaques vaccinated with ALVAC-SIV/gp120 adjuvanted in Alum or MF59.

Project description:The Canarypox/gp120/Alum vaccines decreased the risk of HIV acquisition in humans. We demonstrate here the efficacy of this vaccine regimen also in the SIVmac251 macaque model when we used the alum but not the MF59 adjuvant. Analysis of innate and adaptive cell responses, envelope antibodies Fc profiles and glycoforms demonstrated a lower inflammatory response with alum than MF59. Alum elicited mucosal V2 peptide-specific IgG associated with vaccine efficacy whereas the MF59 induced mucosal V2 peptide-specific IgG associated with increased risk of infection. Alum modulated the expression of 12 genes, 7 of which are part of the RAS pathway, that correlates with vaccine efficacy and were linked to innate responses that preserve mucosal integrity and adaptive mucosal antibody response to V2. Thus, activation of the RAS pathway, preservation of mucosal integrity and mucosal antibody to V2 in concert, reduce the risk of SIVmac251 acquisition. Fifty-four (54) rhesus macaques were randomized into two vaccination groups. One group (n=27) was primed twice with ALVAC-SIV (at week 0 and week 4) and boosted twice with ALVAC-SIV/gp120 in MF59 adjuvant (at week 12 and week 24). The second group (n=27) was primed twice with ALVAC-SIV (at week 0 and week 4) and boosted twice with ALVAC-SIV/gp120 in Alum adjuvant (at week 12 and week 24). Blood samples were taken pre-vaccination, 24 hours after the first prime (post-1st imunization at week 0) and 24 hours after the first boost (post-3rd immunization at week 12). All the samples were taken before SIV challenge. Blood samples were conserved in PAXgene tubes. RNA was extracted and hybridized to Illumina beadchips. technical replicate: P162_P382_post1st, P162_P382_post1st_rep1

2016-05-29 | E-GEOD-72624 | biostudies-arrayexpress

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