Project description:Investigation of whole genome gene expression level changes in leaves of susceptible (MM106) or resistant (ME010) apple genotypes either non-inoculated, inoculated with the wild type strain of Erwinia amylovora (CFBP1430) or the non-pathogenic mutant strain PMV6023. The mutant strain PMV6023 is affected in the biosynthesis of the type 3 secretion system delivering bacterial effectors into apple cells. Leaves were harvested for RNA extraction before inoculation, 6 hours and 24 hours after inoculation.
2021-05-25 | GSE175422 | GEO
Project description:BS-seq and RNA-seq of different apple leaves
Project description:Fire blight (FB) is a bacterial disease affecting plants from Rosaceae family, including apple and pear. FB develops after the infection of Erwinia amylovora, gram-negative enterobacterium, and results in burnt-like damages and wilting, which can affect all organs of the plant. Although the mechanisms underlying disease response in apples are not elucidated yet, it has been well described that FB resistance depends on the rootstock type. The main objective of this work was to identify miRNAs involved in response to bacterial infection in order to better explain apple defense mechanisms against fire blight disease. We performed deep sequencing of eighteen small RNA libraries obtained from inoculated and non-inoculated Gala apple leaves. 233 novel plant mature miRNAs were identified together with their targets and potential role in response to bacterial infection. We identify three apple miRNAs responding to inoculation (mdm-miR168a,b, mdm-miR194C and mdm-miR1392C) as well as miRNAs reacting to bacterial infection in a rootstock-specific manner (miR395 family). Our results provide insights into the mechanisms of fire blight resistance in apple.
Project description:Investigation of whole genome gene expression level changes in leaves of apple seedlings (Golden delicious) 3 days after treatment by Bion 50WG (ASM) versus water.
2024-04-01 | GSE262538 | GEO
Project description:Dual RNA-seq of Gymnosporangium yamadae inoculated apple leaves and mock-inoculated apple leaves at 10 dpi and 30 dpi
Project description:Induction of whole genome gene expression level changes in leaves of grafted apple genotypes exhibiting contrasted QTL architecture (from one to a combination of three QTL) after the inoculation of the pathogenic fungus Venturia inaequalis
Project description:The main objective of this analysis was to sequence the epigenome of the Apple (Malus domestica) doubled haploid 'Golden Delicious' tree. Our secondary objective was to identify differentially methylated regions between DNA purified from leaves and young fruits.